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Wipe sampling procedure coupled to LC-MS/MS analysis for the simultaneous determination of 10 cytotoxic drugs on different surfaces

Nussbaumer, Susanne ; Geiser, Laurent ; Sadeghipour, Farshid ; Hochstrasser, Denis ; Bonnabry, Pascal ; Veuthey, Jean-Luc ; Fleury-Souverain, Sandrine

In: Analytical and Bioanalytical Chemistry, 2012, vol. 402, no. 8, p. 2499-2509

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    Titre
    • Wipe sampling procedure coupled to LC-MS/MS analysis for the simultaneous determination of 10 cytotoxic drugs on different surfaces
    Auteur
    • Nussbaumer, Susanne. Pharmacy, Geneva University Hospitals (HUG), 1211, Geneva 14, Switzerland
    • Geiser, Laurent. Swiss Centre for Applied Human Toxicology, 1211, Geneva 4, Switzerland
    • Sadeghipour, Farshid. Pharmacy, Geneva University Hospitals (HUG), 1211, Geneva 14, Switzerland
    • Hochstrasser, Denis. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne,, 1211, Geneva 4, Switzerland
    • Bonnabry, Pascal. Pharmacy, Geneva University Hospitals (HUG), 1211, Geneva 14, Switzerland
    • Veuthey, Jean-Luc. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne,, 1211, Geneva 4, Switzerland
    • Fleury-Souverain, Sandrine. Pharmacy, Geneva University Hospitals (HUG), 1211, Geneva 14, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Analytical and Bioanalytical Chemistry, 2012, vol. 402, no. 8, p. 2499-2509. Springer-Verlag
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:314559
    Summary
    A simple wipe sampling procedure was developed for the surface contamination determination of ten cytotoxic drugs: cytarabine, gemcitabine, methotrexate, etoposide phosphate, cyclophosphamide, ifosfamide, irinotecan, doxorubicin, epirubicin and vincristine. Wiping was performed using Whatman filter paper on different surfaces such as stainless steel, polypropylene, polystyrol, glass, latex gloves, computer mouse and coated paperboard. Wiping and desorption procedures were investigated: The same solution containing 20% acetonitrile and 0.1% formic acid in water gave the best results. After ultrasonic desorption and then centrifugation, samples were analysed by a validated liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in selected reaction monitoring mode. The whole analytical strategy from wipe sampling to LC-MS/MS analysis was evaluated to determine quantitative performance. The lowest limit of quantification of 10ng per wiping sample (i.e. 0.1ngcm−2) was determined for the ten investigated cytotoxic drugs. Relative standard deviation for intermediate precision was always inferior to 20%. As recovery was dependent on the tested surface for each drug, a correction factor was determined and applied for real samples. The method was then successfully applied at the cytotoxic production unit of the Geneva University Hospitals pharmacy. Figure Wipe sampling procedure for the determination of cytotoxic drugs