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SMB Operation for Three-Fraction Separations: Purification of Plasmid DNA

Paredes, Galatea ; Mazzotti, Marco ; Stadler, Joachim ; Makart, Stefan ; Morbidelli, Massimo

In: Adsorption, 2005, vol. 11, p. 841-845

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    Titre
    • SMB Operation for Three-Fraction Separations: Purification of Plasmid DNA
    Auteur
    • Paredes, Galatea. ETH Swiss Federal Institute of Technology Zurich, Sonneggstrasse 3, CH-8092, Zurich, Switzerland
    • Mazzotti, Marco. ETH Swiss Federal Institute of Technology Zurich, Sonneggstrasse 3, CH-8092, Zurich, Switzerland
    • Stadler, Joachim. Amersham Biosciences Europe GmbH, D-79111, Freiburg, Germany
    • Makart, Stefan. ETH Swiss Federal Institute of Technology Zurich, Sonneggstrasse 3, CH-8092, Zurich, Switzerland
    • Morbidelli, Massimo. ETH Swiss Federal Institute of Technology Zurich, Sonneggstrasse 3, CH-8092, Zurich, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:314398
    Summary
    The Simulated Moving Bed technology is extended to incorporate a cleaning in place step, and it is then applied by exploiting size exclusion chromatography to purify plasmid DNA. Experimental performances are discussed in the light of our theoretical understanding of the SMB behavior