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Overexpression of D-myo-inositol-3-phosphate synthase leads to elevated levels of inositol in Arabidopsis

Smart*, Cheryl ; Flores, Susan

In: Plant Molecular Biology, 1997, vol. 33, no. 5, p. 811-820

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    Title
    • Overexpression of D-myo-inositol-3-phosphate synthase leads to elevated levels of inositol in Arabidopsis
    Author
    • Smart*, Cheryl. Institute of Plant Sciences, Plant Biochemistry and Physiology, Swiss Federal Institute of Technology (ETH) Zürich, Universitätsstrasse 2, CH-8092, Zürich, Switzerland
    • Flores, Susan. Institute of Plant Sciences, Plant Biochemistry and Physiology, Swiss Federal Institute of Technology (ETH) Zürich, Universitätsstrasse 2, CH-8092, Zürich, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Plant Molecular Biology, 1997, vol. 33, no. 5, p. 811-820. Kluwer Academic Publishers
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:313495
    Summary
    In this paper, we report on the generation of transgenic Arabidopsis plants containing elevated levels of the gene product encoding the enzyme catalysing the first committed step in inositol biosynthesis, D-myo-inositol-3-phosphate (Ins3P) synthase. These plants exhibit both an increase in Ins3P synthase activity and an increase in the level of free inositol of over four-fold compared to wild-type plants. Despite these changes, we could detect no significant difference in phenotype in the transgenic plants for a number of characteristics linked with putative functions of inositol and inositol-derived metabolites. Our results indicate that the proposed engineering of inositol metabolism to generate specific plant phenotypes (e.g. salt tolerance) may require the manipulation of several genes, and that Ins3P synthase activity can be manipulated to increase the pool size of free inositol