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Beta-aminobutyric acid-induced resistance in grapevine against downy mildew: involvement of pterostilbene

Slaughter, Ana ; Hamiduzzaman, Mollah ; Gindro, Katia ; Neuhaus, Jean-Marc ; Mauch-Mani, Brigitte

In: European Journal of Plant Pathology, 2008, vol. 122, no. 1, p. 185-195

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    Title
    • Beta-aminobutyric acid-induced resistance in grapevine against downy mildew: involvement of pterostilbene
    Author
    • Slaughter, Ana. Laboratory of Molecular and Cellular Biology, University of Neuchâtel, Rue Emile-Argand 11, Case Postale 158, CH-2009, Neuchâtel, Switzerland
    • Hamiduzzaman, Mollah. Laboratory of Molecular and Cellular Biology, University of Neuchâtel, Rue Emile-Argand 11, Case Postale 158, CH-2009, Neuchâtel, Switzerland
    • Gindro, Katia. Swiss Federal Research Station for Plant Production of Changins-Wädenswil ACW, Case Postale 1060, CH-12, Nyon, Switzerland
    • Neuhaus, Jean-Marc. Laboratory of Molecular and Cellular Biology, University of Neuchâtel, Rue Emile-Argand 11, Case Postale 158, CH-2009, Neuchâtel, Switzerland
    • Mauch-Mani, Brigitte. Laboratory of Molecular and Cellular Biology, University of Neuchâtel, Rue Emile-Argand 11, Case Postale 158, CH-2009, Neuchâtel, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • European Journal of Plant Pathology, 2008, vol. 122, no. 1, p. 185-195. Springer Netherlands
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:313198
    Summary
    BABA, a non-protein amino acid, was used to induce resistance in grapevine against downy mildew. BABA-induced resistance was observed in the susceptible cv. Chasselas as well as in the resistant cv. Solaris. Following BABA treatment, sporulation of Plasmopara viticola was strongly reduced and the accumulation of stilbenes increased with time following infection. Induction of trans-piceide, trans-resveratrol and, more importantly, of trans-ɛ- and trans-δ-viniferin and trans-pterostilbene was observed in BABA-primed Chasselas. On the other hand, induction of trans-resveratrol, trans δ-viniferin and trans-pterostilbene was observed in BABA-primed Solaris. The accumulation of stilbenes in BABA-primed Solaris was much higher than that found in BABA-primed Chasselas. Furthermore, BABA-treatment of Solaris led to a rapid increase in transcript levels of three genes involved in the phenylpropanoid pathway: phenylalanine ammonia lyase, cinnamate-4-hydroxylase and stilbene synthase. BABA-primed Chasselas showed increased transcript levels for cinnamate-4-hydroxylase and stilbene synthase. Here we show that pre-treatment of a susceptible grapevine cultivar with BABA prior to infection with P. viticola primed the accumulation of specific phytoalexins that are undetectable in non-BABA-primed plants. As a result, the susceptible cultivar became more resistant to downy mildew