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A multiplex-system to target 16 male-specific and 15 autosomal genetic markers for orang-utans (genus: Pongo )

Nietlisbach, Pirmin ; Nater, Alexander ; Greminger, Maja ; Arora, Natasha ; Krützen, Michael

In: Conservation Genetics Resources, 2010, vol. 2, p. 153-158

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    Title
    • A multiplex-system to target 16 male-specific and 15 autosomal genetic markers for orang-utans (genus: Pongo )
    Author
    • Nietlisbach, Pirmin. Evolutionary Genetics Group, Anthropological Institute and Museum, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    • Nater, Alexander. Evolutionary Genetics Group, Anthropological Institute and Museum, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    • Greminger, Maja. Evolutionary Genetics Group, Anthropological Institute and Museum, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    • Arora, Natasha. Evolutionary Genetics Group, Anthropological Institute and Museum, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    • Krützen, Michael. Evolutionary Genetics Group, Anthropological Institute and Museum, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:312714
    Summary
    Genetic studies of dispersal on local spatial and short temporal scales require a large number of autosomal microsatellites. However, the study of dispersal over large spatial scales and the resolution of deep evolutionary histories require marker systems that are preferentially inherited through the male or female line. Addressing such questions in endangered orang-utans (genus: Pongo) bears significant relevance to species conservation, as habitat destruction and fragmentation pose a significant threat to the whole genus. Here, we report 16 male-specific markers (nine human-derived microsatellites, six single nucleotide and one insertion-deletion polymorphisms), and 15 novel Pongo-derived autosomal microsatellite loci. All 31 markers can be amplified in four multiplex polymerase chain reactions even in DNA derived from faecal material. The markers can be applied to studying a wide range of important questions in this genus, such as conservation genetics, social structure, phylogeny and phylogeography