Identification of phytotoxins from Botryosphaeria obtusa , a pathogen of black dead arm disease of grapevine

Djoukeng, Jules ; Polli, Suzanna ; Larignon, Philippe ; Abou-Mansour, Eliane

In: European Journal of Plant Pathology, 2009, vol. 124, no. 2, p. 303-308

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    Summary
    A bioassay-guided fractionation of a culture filtrate of Botryosphaeria obtusa led to the isolation of four dihydroisocoumarins, named mellein 1, 4-hydroxymellein 2, 7-hydroxymellein 3 and the new 4,7-dihydroxymellein 4. LC-UV-DAD-MS analysis of vine wood infected by B. obtusa revealed the presence of mellein (1). Botryosphaeria obtusa was also able to oxidise wood δ -resveratrol into the dimer delta-viniferin. The structures of isolated phytotoxins were established on the basis of IR, MS, 1D and 2D NMR