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Improved detection of long-range residual dipolar couplings in weakly aligned samples by Lee-Goldburg decoupling of homonuclear dipolar truncation

Jensen, Pernille ; Sass, Hans-Jürgen ; Grzesiek, Stephan

In: Journal of Biomolecular NMR, 2004, vol. 30, no. 4, p. 443-450

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    Titre
    • Improved detection of long-range residual dipolar couplings in weakly aligned samples by Lee-Goldburg decoupling of homonuclear dipolar truncation
    Auteur
    • Jensen, Pernille. Division of Structural Biology, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland
    • Sass, Hans-Jürgen. Division of Structural Biology, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland
    • Grzesiek, Stephan. Division of Structural Biology, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056, Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of Biomolecular NMR, 2004, vol. 30, no. 4, p. 443-450. Kluwer Academic Publishers
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:310742
    Summary
    Homonuclear 1H residual dipolar couplings (RDCs) truncate the evolution of transverse 1H magnetization of weakly aligned molecules in high-resolution NMR experiments. This leads to losses in sensitivity or resolution in experiments that require extended 1H evolution times. Lee-Goldburg decoupling schemes have been shown to remove the effects of homonuclear dipolar couplings, while preserving chemical shift evolution in a number of solid-state NMR applications. Here, it is shown that the Lee-Goldburg sequence can be effectively incorporated into INEPT- or HMQC-type transfer schemes in liquid state weak alignment experiments in order to increase the efficiency of the magnetization transfer. The method is applied to the sensitive detection of 1HN-13C long-range RDCs in a three-dimensional HCN experiment. As compared to a conventional HCN experiment, an average sensitivity increase by a factor of 2.4 is obtained for a sample of weakly aligned protein G. This makes it possible to detect 170 long-range 1HN-13C RDCs for distances up to 4.9 Å