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Buccal swabs allow efficient and reliable microsatellite genotyping in amphibians

Broquet, Thomas ; Berset-Braendli, Laura ; Emaresi, Guillaume ; Fumagalli, Luca

In: Conservation Genetics, 2007, vol. 8, no. 2, p. 509-511

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    Titre
    • Buccal swabs allow efficient and reliable microsatellite genotyping in amphibians
    Auteur
    • Broquet, Thomas. Department of Ecology and Evolution, Laboratory for Conservation Biology, University of Lausanne, CH-1015, Lausanne, Switzerland
    • Berset-Braendli, Laura. Department of Ecology and Evolution, Laboratory for Conservation Biology, University of Lausanne, CH-1015, Lausanne, Switzerland
    • Emaresi, Guillaume. Department of Ecology and Evolution, Laboratory for Conservation Biology, University of Lausanne, CH-1015, Lausanne, Switzerland
    • Fumagalli, Luca. Department of Ecology and Evolution, Laboratory for Conservation Biology, University of Lausanne, CH-1015, Lausanne, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Conservation Genetics, 2007, vol. 8, no. 2, p. 509-511. Kluwer Academic Publishers
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:310728
    Summary
    Buccal swabs have recently been used as a minimally invasive sampling method in genetic studies of wild populations, including amphibian species. Yet it is not known to date what is the level of reliability for microsatellite genotypes obtained using such samples. Allelic dropout and false alleles may affect the genotyping derived from buccal samples. Here we quantified the success of microsatellite amplification and the rates of genotyping errors using buccal swabs in two amphibian species, the Alpine newt Triturus alpestris and the Green tree frog Hyla arborea, and we estimated two important parameters for downstream analyses, namely the number of repetitions required to achieve typing reliability and the probability of identity among genotypes. Amplification success was high, and only one locus tested required two to three repetitions to achieve reliable genotypes, showing that buccal swabbing is a very efficient approach allowing good quality DNA retrieval. This sampling method which allows avoiding the controversial toe-clipping will likely prove very useful in the context of amphibian conservation