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Development of polymorphic microsatellite markers for the critically endangered and endemic Indian dipterocarp, Vateria indica L. (Dipterocarpaceae)

Ismail, Sascha ; Buser, Andres ; Uma Shaanker, R. ; Ravikanth, G. ; Ghazoul, Jaboury ; Kettle, Chris

In: Conservation Genetics Resources, 2013, vol. 5, no. 2, p. 465-467

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    Titre
    • Development of polymorphic microsatellite markers for the critically endangered and endemic Indian dipterocarp, Vateria indica L. (Dipterocarpaceae)
    Auteur
    • Ismail, Sascha. Department of Environmental Systems Science, ITES-Ecosystem Management, ETH Zurich, Universitaetsstrasse 16, 8092, Zurich, Switzerland
    • Buser, Andres. Ecogenics GmbH, Grabenstrasse 11a, Schlieren, 8952, Zurich, Switzerland
    • Uma Shaanker, R.. Department of Crop Physiology and School of Ecology and Conservation, University of Agricultural Sciences, Bangalore, Karnataka, India
    • Ravikanth, G.. Department of Conservation Genetics, Ashoka Trust for Research in Ecology and the Environment, Bangalore, Karnataka, India
    • Ghazoul, Jaboury. Department of Environmental Systems Science, ITES-Ecosystem Management, ETH Zurich, Universitaetsstrasse 16, 8092, Zurich, Switzerland
    • Kettle, Chris. Department of Environmental Systems Science, ITES-Ecosystem Management, ETH Zurich, Universitaetsstrasse 16, 8092, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Conservation Genetics Resources, 2013, vol. 5, no. 2, p. 465-467. Springer Netherlands
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:310546
    Summary
    Vateria indica (Dipterocarpaceae) is an economically and ecologically important canopy tree endemic to the Western Ghats, India. The species has undergone extensive habitat loss and overexploitation and is therefore listed as ‘critically endangered' on the 2012 IUCN Red List. We developed ten polymorphic microsatellite loci for V. indica. In addition, we confirm cross amplification and variation in two loci isolated from the closely related but geographically disjunct species Vateriopsis seychellarum, previously published by Finger et al. Conserv Genet Resour, 2 (S1):309-311, (2010). The twelve microsatellite primers screened on 48 adult samples of V. indica had 5-11 alleles per locus (mean of 8.5 per locus) with an average polymorphic information content of 0.64 across loci. Expected heterozygosity ranged from 0.44 to 0.84. These markers will enable us to quantify population genetic diversity in habitat fragments and to study fine scale spatial genetic structure and contemporary gene flow