Fulltext

Real-time broad-range PCR versus blood culture. A prospective pilot study in pediatric cancer patients with fever and neutropenia

Ammann, Roland ; Zucol, Franziska ; Aebi, Christoph ; Niggli, Felix ; Kühne, Thomas ; Nadal, David

In: Supportive Care in Cancer, 2007, vol. 15, no. 6, p. 637-641

Ajouter à la liste personnelle
    Titre
    • Real-time broad-range PCR versus blood culture. A prospective pilot study in pediatric cancer patients with fever and neutropenia
    Auteur
    • Ammann, Roland. Pediatric Hematology/Oncology, Department of Pediatrics, University of Bern, CH-3010, Bern, Switzerland
    • Zucol, Franziska. Division of Infectious Diseases, University Children's Hospital, University of Zurich, Zurich, Switzerland
    • Aebi, Christoph. Institute of Infectious Diseases, University of Bern, Bern, Switzerland
    • Niggli, Felix. Division of Oncology, University Children's Hospital, University of Zurich, Zurich, Switzerland
    • Kühne, Thomas. Pediatric Hematology/Oncology, University Children's Hospital, University of Basel, Basel, Switzerland
    • Nadal, David. Division of Infectious Diseases, University Children's Hospital, University of Zurich, Zurich, Switzerland
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:309940
    Summary
    Materials and methods: In a pilot study, results of real-time broad-range (16S rRNA) polymerase chain reaction (PCR) performed on 45 blood samples of pediatric cancer patients with fever and neutropenia were compared with blood culture results. Results: The PCR assay used, having proven a high sensitivity in artificially spiked blood samples, was positive in only three of ten blood culture-positive samples, and it was positive in 10 of 35 (29%) culture-negative samples. Conclusion: This broad-range PCR assay, which may identify not-grown bacteria potentially contributing to fever, needs improvement in sensitivity, and different reasons for positive PCR in negative blood culture samples need to be assessed before clinical application