000030554 001__ 30554
000030554 005__ 20131002114017.0
000030554 0248_ $$aoai:doc.rero.ch:20121108083445-JT$$punifr$$ppostprint$$prero_explore$$zcdu34$$zthesis_urn$$zcdu57$$zreport$$zthesis$$zbook$$zjournal$$zcdu16$$zpreprint$$zcdu1$$zdissertation
000030554 041__ $$aeng
000030554 080__ $$a57
000030554 100__ $$aHuser, Annina$$uDepartment of Biology, University of Fribourg, Switzerland
000030554 245__ $$9eng$$aThe serotonergic central nervous system of the drosophila larva: anatomy and behavioral function
000030554 269__ $$c2012-10-17
000030554 520__ $$9eng$$aThe Drosophila larva has turned into a particularly simple model system for studying the neuronal basis of innate behaviors and higher brain functions. Neuronal networks involved in olfaction, gustation, vision and learning and memory have been described during the last decade, often up to the single-cell level. Thus, most of these sensory networks are substantially defined, from the sensory level up to third-order neurons. This is especially true for the olfactory system of the larva. Given the wealth of genetic tools in Drosophila it is now possible to address the question how modulatory systems interfere with sensory systems and affect learning and memory. Here we focus on the serotonergic system that was shown to be involved in mammalian and insect sensory perception as well as learning and memory. Larval studies suggested that the serotonergic system is involved in the modulation of olfaction, feeding, vision and heart rate regulation. In a dual anatomical and behavioral approach we describe the basic anatomy of the larval serotonergic system, down to the single-cell level. In parallel, by expressing apoptosis-inducing genes during embryonic and larval development, we ablate most of the serotonergic neurons within the larval central nervous system. When testing these animals for naïve odor, sugar, salt and light perception, no profound phenotype was detectable; even appetitive and aversive learning was normal. Our results provide the first comprehensive description of the neuronal network of the larval serotonergic system. Moreover, they suggest that serotonin per se is not necessary for any of the behaviors tested. However, our data do not exclude that this system may modulate or fine-tune a wide set of behaviors, similar to its reported function in other insect species or in mammals. Based on our observations and the availability of a wide variety of genetic tools, this issue can now be addressed.
000030554 700__ $$aRohwedder, Astrid$$uDepartment of Biology, University of Fribourg, Switzerland - Department of Biology, University of Konstanz, Germany
000030554 700__ $$aApostolopoulou, Anthi A.$$uDepartment of Biology, University of Fribourg, Switzerland - Department of Biology, University of Konstanz, Germany
000030554 700__ $$aWidmann, Annekathrin$$uDepartment of Biology, University of Fribourg, Switzerland - Department of Biology, University of Konstanz, Germany
000030554 700__ $$aPfitzenmaier, Johanna E. R.$$uDepartment of Biology, University of Fribourg, Switzerland - Department of Biology, University of Konstanz, Germany
000030554 700__ $$aMaiolo, Elena M.$$uDepartment of Biology, University of Fribourg, Switzerland
000030554 700__ $$aSelcho, Mareike$$uNeurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, Germany
000030554 700__ $$aPauls, Dennis$$uNeurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg, Germany
000030554 700__ $$aEssen, Alina von$$uDepartment of Biology, University of Fribourg, Switzerland
000030554 700__ $$aGupta, Tripti$$uDepartment of Biology, University of Fribourg, Switzerland
000030554 700__ $$aSprecher, Simon G.$$uDepartment of Biology, University of Fribourg, Switzerland
000030554 700__ $$aBirman, Serge$$uGenetic and Physiopathology of Neurotransmission, ESPCI Neurobiology Unit, Centre National de la Recherche Scientifique, Paris, France
000030554 700__ $$aRiemensperger, Thomas$$uMolecular Neurobiology of Behavior, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Goettingen, Germany
000030554 700__ $$aStocker, Reinhard F.$$uDepartment of Biology, University of Fribourg, Switzerland
000030554 700__ $$aThum, Andreas S.$$uDepartment of Biology, University of Fribourg, Switzerland - Department of Biology, University of Konstanz, Germany
000030554 773__ $$g2012/7/10/e47518$$tPLoS ONE
000030554 775__ $$gPublished version$$ohttp://dx.doi.org/10.1371/journal.pone.0047518
000030554 8564_ $$fhus_scn.pdf$$qapplication/pdf$$s2961294$$uhttp://doc.rero.ch/record/30554/files/hus_scn.pdf$$yorder:1$$zpdf
000030554 918__ $$aFaculté des sciences$$bDécanat, Ch. du Musée 6A, 1700 Fribourg$$cBiologie
000030554 919__ $$aUniversité de Fribourg$$bFribourg$$ddoc.support@rero.ch
000030554 980__ $$aPOSTPRINT$$bUNIFR$$fART_JOURNAL
000030554 990__ $$a20121108083445-JT