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000030532 005__ 20150420164422.0
000030532 0248_ $$aoai:doc.rero.ch:20121031095409-TQ$$punifr$$ppostprint$$prero_explore$$particle$$zcdu34$$zthesis_urn$$zreport$$zthesis$$zbook$$zjournal$$zcdu16$$zpreprint$$zcdu1$$zdissertation$$zcdu53
000030532 041__ $$aeng
000030532 080__ $$a53
000030532 100__ $$aBolisetty, Sreenath$$uETH Zurich, Food and Soft Materials Laboratory, Department of Health Science and Technology, Switzerland
000030532 245__ $$9eng$$aGelation, phase behavior, and dynamics of β-Lactoglobulin amyloid fibrils at varying concentrations and ionic strengths
000030532 269__ $$c2012-08-27
000030532 520__ $$9eng$$aWe have investigated the thermodynamic and dynamic behavior of multistranded β-lactoglobulin protein fibrils in water, by combining static, dynamic, and depolarized dynamic light scattering (SLS, DLS, DDLS), small angle neutron scattering (SANS), rheology, and cryogenic transmission electron microscopy (cryo-TEM). We focus on the region of the phase diagram at which ionic strength and concentration changes induce transitions in gelation and lyotropic liquid crystalline behavior. An increase in ionic strength, induced by NaCl salt, progressively causes the phase transitions from nematic (N) to gel (G) phases; a further increase causes the transition to a translucent phase and to a macroscopic phase separation, respectively. An increase in fibril concentration induces first a phase transition from an isotropic (I) to a nematic phase (N); a further increase induces the formation of a gel phase. The protein gel strength is investigated by rheology measurements. SANS and osmotic compressibility calculated by SLS measurements clearly capture the main features of the IN transition of β-lactoglobulin protein fibrils. The form and structure factors measured by scattering experiments are analyzed by the polymer reference interaction site model (PRISM). Dynamics of the protein fibrils at different concentrations, measured by polarized and depolarized dynamic light scattering, show both individual and collective diffusion after the isotropic–nematic transition. Above this transition, cryo-TEM images further demonstrate the alignment of the protein fibrils, which is quantified by a 2D order parameter. This work discusses comprehensively, both experimentally and theoretically, the thermodynamics and dynamic features of β-lactoglobulin amyloid fibrils in a vast region of the concentration–ionic strength phase diagram.
000030532 700__ $$aHarnau, Ludger$$uMax-Planck-Institut für Intelligente Systeme, Stuttgart, Germany - Institut für Theoretische und Angewandte Physik, Universität Stuttgart, Germany
000030532 700__ $$aJung, Jin-mi$$uDepartment of Physics, University of Fribourg, Switzerland
000030532 700__ $$aMezzenga, Raffaele$$uETH Zurich, Food and Soft Materials Laboratory, Department of Health Science and Technology, Switzerland
000030532 773__ $$g2012/13/10/3241–3252$$tBiomacromolecules
000030532 775__ $$gPublished version$$ohttp://dx.doi.org/10.1021/bm301005w
000030532 8564_ $$fjun_gpb.pdf$$qapplication/pdf$$s1160271$$uhttp://doc.rero.ch/record/30532/files/jun_gpb.pdf$$yorder:1$$zpdf
000030532 8564_ $$fjun_gpb_sm.pdf$$qapplication/pdf$$s837780$$uhttp://doc.rero.ch/record/30532/files/jun_gpb_sm.pdf$$yorder:2$$zSupplementary material
000030532 918__ $$aFaculté des sciences$$bDécanat, Ch. du Musée 6A, 1700 Fribourg$$cPhysique
000030532 919__ $$aUniversité de Fribourg$$bFribourg$$ddoc.support@rero.ch
000030532 980__ $$aPOSTPRINT$$bUNIFR$$fART_JOURNAL
000030532 990__ $$a20121031095409-TQ