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The food additive vanillic acid controls transgene expression in mammalian cells and mice

Gitzinger, Marc ; Kemmer, Christian ; Fluri, David A. ; Daoud El-Baba, Marie ; Weber, Wilfried ; Fussenegger, Martin

In: Nucleic Acids Research, 2012, vol. 40, no. 5, p. e37-e37

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    Titre
    • The food additive vanillic acid controls transgene expression in mammalian cells and mice
    Auteur
    • Gitzinger, Marc. Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada, Université de Lyon, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France, Faculty of Biology and BIOSS Center for Biological Signaling Studies, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany
    • Kemmer, Christian. Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada, Université de Lyon, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France, Faculty of Biology and BIOSS Center for Biological Signaling Studies, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany
    • Fluri, David A.. Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada, Université de Lyon, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France, Faculty of Biology and BIOSS Center for Biological Signaling Studies, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany
    • Daoud El-Baba, Marie. Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada, Université de Lyon, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France, Faculty of Biology and BIOSS Center for Biological Signaling Studies, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany
    • Weber, Wilfried. Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada, Université de Lyon, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France, Faculty of Biology and BIOSS Center for Biological Signaling Studies, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany
    • Fussenegger, Martin. Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada, Université de Lyon, 43 Boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France, Faculty of Biology and BIOSS Center for Biological Signaling Studies, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Nucleic Acids Research, 2012, vol. 40, no. 5, p. e37-e37. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:303886
    Summary
    Trigger-inducible transcription-control devices that reversibly fine-tune transgene expression in response to molecular cues have significantly advanced the rational reprogramming of mammalian cells. When designed for use in future gene- and cell-based therapies the trigger molecules have to be carefully chosen in order to provide maximum specificity, minimal side-effects and optimal pharmacokinetics in a mammalian organism. Capitalizing on control components that enable Caulobacter crescentus to metabolize vanillic acid originating from lignin degradation that occurs in its oligotrophic freshwater habitat, we have designed synthetic devices that specifically adjust transgene expression in mammalian cells when exposed to vanillic acid. Even in mice transgene expression was robust, precise and tunable in response to vanillic acid. As a licensed food additive that is regularly consumed by humans via flavoured convenience food and specific fresh vegetable and fruits, vanillic acid can be considered as a safe trigger molecule that could be used for diet-controlled transgene expression in future gene- and cell-based therapies