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Memory TCR repertoires analyzed long-term reflect those selected during the primary response

Walker, Paul R. ; Wilson, Anne ; Bucher, Philipp ; Maryanski, Janet L.

In: International Immunology, 1996, vol. 8, no. 7, p. 1131-1138

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    Titre
    • Memory TCR repertoires analyzed long-term reflect those selected during the primary response
    Auteur
    • Walker, Paul R.. Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne 1066 Epalinges, Switzerland
    • Wilson, Anne. Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne 1066 Epalinges, Switzerland
    • Bucher, Philipp. Swiss Institute for Experimental Cancer Research 1066 Epalinges, Switzerland
    • Maryanski, Janet L.. Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne 1066 Epalinges, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • International Immunology, 1996, vol. 8, no. 7, p. 1131-1138. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:303795
    Summary
    Normal T cell repertoire selection and evolution in antigen-specific responses is poorly understood. We have recently described an MHC class I-restricted response characterized by an overwhelming expansion of CD8 cells expressing a Vβ10 TCR, thus allowing the identification of antigen-selected cells directly ex vivo. Our present strategy to follow the overall TCR repertoire selection was to monitor the expression of a particular TCR α chain (Vα8) on antigen-selected Vβ10+ cells by four-color flow cytometry. We demonstrate that while there is substantial variation among the responder mice in Vα8 usage, the repertoires of individual animals remain relatively stable over long periods of time (>1 year), with or without repeated antigenic challenge. Thus if any evolution of this response occurs upon re-exposure to antigen, it would appear not to skew the TCR repertoire established during the primary response