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Uterus and endometrium: Regeneration processes in rabbit endometrium: a photodynamic therapy model

Wyss, P. ; Steiner, R. ; Liaw, L.H. ; Wyss, M.T. ; Ghazarians, A. ; Berns, M.W. ; Tromberg, B.J. ; Tadir, Y.

In: Human Reproduction, 1996, vol. 11, no. 9, p. 1992-1997

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    Titre
    • Uterus and endometrium: Regeneration processes in rabbit endometrium: a photodynamic therapy model
    Auteur
    • Wyss, P.. Beckman Laser Institute and Medical Clinic, Departments of Obstetrics and Gynecology, University of California Irvine, CA, USA
    • Steiner, R.. Beckman Laser Institute and Medical Clinic, Departments of Obstetrics and Gynecology, University of California Irvine, CA, USA
    • Liaw, L.H.. Beckman Laser Institute and Medical Clinic, Departments of Obstetrics and Gynecology, University of California Irvine, CA, USA
    • Wyss, M.T.. Beckman Laser Institute and Medical Clinic, Departments of Obstetrics and Gynecology, University of California Irvine, CA, USA
    • Ghazarians, A.. Beckman Laser Institute and Medical Clinic, Departments of Obstetrics and Gynecology, University of California Irvine, CA, USA
    • Berns, M.W.. Beckman Laser Institute and Medical Clinic, Departments of Obstetrics and Gynecology, University of California Irvine, CA, USA
    • Tromberg, B.J.. Beckman Laser Institute and Medical Clinic, Departments of Obstetrics and Gynecology, University of California Irvine, CA, USA
    • Tadir, Y.. Beckman Laser Institute and Medical Clinic, Departments of Obstetrics and Gynecology, University of California Irvine, CA, USA
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:303466
    Summary
    The origin and process of regeneration in rabbit endo metrium was evaluated following photodynamic epithelial destruction using topically applied anilnolevulinic acid (ALA). Selective destruction of endometrial epithelium was performed using photodynamic therapy (PDT). ALA was diluted to 200 mg/ml dextran 70 shortly prior to adininistra tion. A volume of 1.2 ml was injected into the left uterus. Intrauterine illumination (wavelength 630 nm, light dose 40-80 J/cm2was performed 3 h after drug administration. Tissue morphology was evaluated by light and scanning electron microscopy 1, 3, 7 and 28 days post-treatment (three animals at each dine-point). Regeneration of the endometriwn following epithelial ablation by PDT was fully activated after 24 h and was completed after 72 h. Endometrial surface generation occurred by proliferation, originating primarily in deeper regions of the glands. Findings from our morphological follow-up study support the origin of endometrial regeneration being mainly from undifferentiated stem cells and residual glandular epithelium