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Detection of Exogenous GHB in Blood by Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry: Implications in Postmortem Toxicology

Saudan, Christophe ; Augsburger, Marc ; Kintz, Pascal ; Saugy, Martial ; Mangin, Patrice

In: Journal of Analytical Toxicology, 2005, vol. 29, no. 8, p. 777-781

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    Titel
    • Detection of Exogenous GHB in Blood by Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry: Implications in Postmortem Toxicology
    Autor
    • Saudan, Christophe. Laboratoire Suisse d'Analyse du Dopage, Institut Universitaire de Médecine Légale, Rue du Bugnon 21, 1005 Lausanne, Switzerland
    • Augsburger, Marc. Laboratoire de Toxicologie et de Chimie Forensiques, Institut Universitaire de Médecine Légale, Rue du Bugnon 21, 1005 Lausanne, Switzerland
    • Kintz, Pascal. Institut de Médecine Légale, Université Louis Pasteur (ULP), 11 rue Humann, 67000 Strasbourg, France
    • Saugy, Martial. Laboratoire Suisse d'Analyse du Dopage, Institut Universitaire de Médecine Légale, Rue du Bugnon 21, 1005 Lausanne, Switzerland
    • Mangin, Patrice. Laboratoire Suisse d'Analyse du Dopage, Institut Universitaire de Médecine Légale, Rue du Bugnon 21, 1005 Lausanne, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Journal of Analytical Toxicology, 2005, vol. 29, no. 8, p. 777-781. Oxford University Press
    Andere elektronische Ausgabe
    Klassifikation
    • Gesundheit
    OAI-PMH-ID
    • oai:doc.rero.ch:302859
    Summary
    Because GHB (γ-hydroxybutyrate) is present in both blood and urine of the general population, toxicologists must be able to discriminate between endogenous levels and a concentration resulting from exposure. In this paper, we propose a procedure for the detection of exogenous GHB in blood by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Following liquid-liquid and solid-phase extractions, GHB is derivatized to GHB di-TMS before analysis by GC-C-IRMS. Significant differences in the carbon isotopic ratio (Δδ13C-values > 13.5‰) were found between endogenous and synthetic GHB. Indeed, for postmortem blood samples with different GHB concentrations (range: 13.8-86.3 mg/L), we have obtained GHB δ13C-values ranging from −20.6 to −24.7‰, whereas δ13C-values for the GHB from police seizure were in the range −38.2 to −50.2‰. In contrast to the use of cut-off concentrations for positive postmortem blood GHB concentrations, this method should provide an unambiguous indication of the drug origin