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Meropenem/colistin synergy testing for multidrug-resistant Acinetobacter baumannii strains by a two-dimensional gradient technique applicable in routine microbiology

van Belkum, Alex ; Halimi, Diane ; Bonetti, Eve-Julie ; Renzi, Gesuele ; Cherkaoui, Abdessalam ; Sauvonnet, Véronique ; Martelin, Roland ; Durand, Géraldine ; Chatellier, Sonia ; Zambardi, Gilles ; Engelhardt, Anette ; Karlsson, Åsa ; Schrenzel, Jacques

In: Journal of Antimicrobial Chemotherapy, 2015, vol. 70, no. 1, p. 167-172

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    Title
    • Meropenem/colistin synergy testing for multidrug-resistant Acinetobacter baumannii strains by a two-dimensional gradient technique applicable in routine microbiology
    Author
    • van Belkum, Alex. Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France
    • Halimi, Diane. Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France
    • Bonetti, Eve-Julie. Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland
    • Renzi, Gesuele. Bacteriology Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland
    • Cherkaoui, Abdessalam. Bacteriology Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland
    • Sauvonnet, Véronique. Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France
    • Martelin, Roland. Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France
    • Durand, Géraldine. Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France
    • Chatellier, Sonia. Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France
    • Zambardi, Gilles. Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France
    • Engelhardt, Anette. AB BIODISK Research Laboratories, Solna, Sweden
    • Karlsson, Åsa. Microbiology R&D, Microbiology Unit, bioMérieux SA, La Balme Les Grottes, France
    • Schrenzel, Jacques. Genomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospital (HUG), Geneva, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Journal of Antimicrobial Chemotherapy, 2015, vol. 70, no. 1, p. 167-172. Oxford University Press
    Other Version
    OAI-PMH Identifier
    • oai:doc.rero.ch:302368
    Summary
    Objectives Precise assessment of potential therapeutic synergy, antagonism or indifference between antimicrobial agents currently depends on time-consuming and hard-to-standardize in vitro chequerboard titration methods. We here present a method based on a novel two-dimensional antibiotic gradient technique named Xact™. Methods We used a test comprising a combination of perpendicular gradients of meropenem and colistin in a single quadrant. We compared test outcomes with those obtained with classical chequerboard microbroth dilution testing in a study involving 27 unique strains of multidrug-resistant Acinetobacter baumannii from diverse origins. Results We were able to demonstrate 92% concordance between the new technology and classical chequerboard titration using the A. baumannii collection. Two strains could not be analysed by Xact™ due to their out-of-range MIC of meropenem (>128 mg/L). Conclusions The new test was shown to be diagnostically useful, easy to implement and less labour intensive than the classical method