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Transcription-coupled deposition of histone modifications during MHC class II gene activation

Rybtsova, Natalia ; Leimgruber, Elisa ; Seguin-Estévez, Queralt ; Dunand-Sauthier, Isabelle ; Krawczyk, Michal ; Reith, Walter

In: Nucleic Acids Research, 2007, vol. 35, no. 10, p. 3431-3441

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    Titre
    • Transcription-coupled deposition of histone modifications during MHC class II gene activation
    Auteur
    • Rybtsova, Natalia. Department of Pathology and Immunology, University of Geneva Medical School, 1 rue Michel-Servet, CH-1211, Geneva, Switzerland
    • Leimgruber, Elisa. Department of Pathology and Immunology, University of Geneva Medical School, 1 rue Michel-Servet, CH-1211, Geneva, Switzerland
    • Seguin-Estévez, Queralt. Department of Pathology and Immunology, University of Geneva Medical School, 1 rue Michel-Servet, CH-1211, Geneva, Switzerland
    • Dunand-Sauthier, Isabelle. Department of Pathology and Immunology, University of Geneva Medical School, 1 rue Michel-Servet, CH-1211, Geneva, Switzerland
    • Krawczyk, Michal. Department of Pathology and Immunology, University of Geneva Medical School, 1 rue Michel-Servet, CH-1211, Geneva, Switzerland
    • Reith, Walter. E-mail: walter.reith@medecine.unige.ch
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Nucleic Acids Research, 2007, vol. 35, no. 10, p. 3431-3441. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:301552
    Summary
    Posttranslational histone modifications associated with actively expressed genes are generally believed to be introduced primarily by histone-modifying enzymes that are recruited by transcription factors or their associated co-activators. We have performed a comprehensive spatial and temporal analyses of the histone modifications that are deposited upon activation of the MHC class II gene HLA-DRA by the co-activator CIITA. We find that transcription-associated histone modifications are introduced during two sequential phases. The first phase precedes transcription initiation and is characterized exclusively by a rapid increase in histone H4 acetylation over a large upstream domain. All other modifications examined, including the acetylation and methylation of several residues in histone H3, are restricted to short regions situated at or within the 5′ end of the gene and are established during a second phase that is concomitant with ongoing transcription. This second phase is completely abrogated when elongation by RNA polymerase II is blocked. These results provide strong evidence that transcription elongation can play a decisive role in the deposition of histone modification patterns associated with inducible gene activation