Fulltext

Monoclonal antibodies raised against membrane glycoproteins from mouse brain recognize N-linked oligomannosidic glycans

Schmitz, Brigitte ; Peter-Katalinic, Jasna ; Egge, Heinz ; Schachner, Melitta

In: Glycobiology, 1993, vol. 3, no. 6, p. 609-617

Add to personal list
    Title
    • Monoclonal antibodies raised against membrane glycoproteins from mouse brain recognize N-linked oligomannosidic glycans
    Author
    • Schmitz, Brigitte. Department of Neurobiology, Swiss Federal Institute of Technology Hōnggerberg, 8093 Zūrich Switzerland
    • Peter-Katalinic, Jasna. Institute of Physiological Chemistry, Nuβallee 11, University of Bonn 5300 Bonn, FRG
    • Egge, Heinz. Institute of Physiological Chemistry, Nuβallee 11, University of Bonn 5300 Bonn, FRG
    • Schachner, Melitta. Department of Neurobiology, Swiss Federal Institute of Technology Hōnggerberg, 8093 Zūrich Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:301315
    Summary
    Monoclonal L3 and L4 antibodies have been shown to recognize carbohydrate epitopes on several neural cell adhesion molecules; these epitopes can be released by treatment with endoglycosidase H. In the present study, we have identified the oligosaccharides released by endoglycosidase H from the cell adhesion molecules AMOG and L1 by fast-atom bombardment mass spectrometry as being solely of the oligomannosidic type. Using neoglycolipids of oligomannosidc glycans, we also report that both antibodies show the highest reactivity with the α-manno-pentaose Manα1-3-[Manα1-6(Manα1-3)Manα1-6]-Man, but decreasing reactivity with the α-manno-hexaose, heptaose, octaose and nonaose glycans. Thus, to our knowledge, we describe here for the first time monoclonal antibodies recognizing N-glycosidically linked oligomannosidic glycans