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Pharmaceutical Applications on Columns Packed with Sub-2 µm Particles

Russo, Rossana ; Guillarme, Davy ; Nguyen, Dao T.-T ; Bicchi, Carlo ; Rudaz, Serge ; Veuthey, Jean-Luc

In: Journal of Chromatographic Science, 2008, vol. 46, no. 3, p. 199-208

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    Title
    • Pharmaceutical Applications on Columns Packed with Sub-2 µm Particles
    Author
    • Russo, Rossana. Laboratory of Phytoanalysis, Dipartimento di Scienza e Tecnologia del Farmaco, Turin University, Via P. Giuria 9-10125 Turin - Italy
    • Guillarme, Davy. Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland
    • Nguyen, Dao T.-T. Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland
    • Bicchi, Carlo. Laboratory of Phytoanalysis, Dipartimento di Scienza e Tecnologia del Farmaco, Turin University, Via P. Giuria 9-10125 Turin - Italy
    • Rudaz, Serge. Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland
    • Veuthey, Jean-Luc. Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, 1211 Geneva 4, Switzerland
    Document Type
    • Postprint
    Language
    • English
    Published in
    • Journal of Chromatographic Science, 2008, vol. 46, no. 3, p. 199-208. Oxford University Press
    Other Version
    Classification
    • Health
    OAI-PMH Identifier
    • oai:doc.rero.ch:300681
    Summary
    During the last few years, there has been a great interest in the development of fast liquid chromatography. Among the reported approaches, the reduction of the particle size to attain sub-2µm diameter represents a good solution for achieving both increased separation power and faster analysis time. This paper demonstrates the chromatographic performance of such supports using plateheight curves and reveals the possibility for obtaining ultra-fast or highly efficient separations, according to the column geometry and system pressure limitations. The stability of these columns is initially evaluated using a system suitability experiment. The chromatographic performance remains stable in terms of retention, efficiency, and pressure for more than 1700 injections with pressure conditions ranging from 200 to 800 bar. Several fast and ultra-fast pharmaceutical applications are reported. In isocratic mode, a 5- to 10-fold reduction in analysis time is obtained with limited influence on efficiency and resolution. The run time is further reduced by 30-fold with the shorter available columns (i.e., 30 mm length). In gradient mode, the separation of a complex mixture containing an active pharmaceutical compound and related impurities is significantly improved with column length equal to 100 mm, to increase peak capacity and resolution