Fulltext

Anti-Apolipoprotein A-1 auto-antibodies are active mediators of atherosclerotic plaque vulnerability

Montecucco, Fabrizio ; Vuilleumier, Nicolas ; Pagano, Sabrina ; Lenglet, Sébastien ; Bertolotto, Maria ; Braunersreuther, Vincent ; Pelli, Graziano ; Kovari, Enikö ; Pane, Bianca ; Spinella, Giovanni ; Pende, Aldo ; Palombo, Domenico ; Dallegri, Franco ; Mach, François ; Roux-Lombard, Pascale

In: European Heart Journal, 2011, vol. 32, no. 4, p. 412-421

Ajouter à la liste personnelle
    Titre
    • Anti-Apolipoprotein A-1 auto-antibodies are active mediators of atherosclerotic plaque vulnerability
    Auteur
    • Montecucco, Fabrizio. Division of Cardiology, Faculty of Medicine, Foundation for Medical Researches, Geneva University Hospitals, avenue de la Roseraie 64, 1211 Geneva 4, Switzerland
    • Vuilleumier, Nicolas. Division of Laboratory Medicine, Department of Genetics and Laboratory Medicine, Geneva University Hospitals, Switzerland
    • Pagano, Sabrina. Division of Laboratory Medicine, Department of Genetics and Laboratory Medicine, Geneva University Hospitals, Switzerland
    • Lenglet, Sébastien. Division of Cardiology, Faculty of Medicine, Foundation for Medical Researches, Geneva University Hospitals, avenue de la Roseraie 64, 1211 Geneva 4, Switzerland
    • Bertolotto, Maria. First Medical Clinic, Laboratory of Phagocyte Physiopathology and Inflammation, Department of Internal Medicine, University of Genoa, Italy
    • Braunersreuther, Vincent. Division of Cardiology, Faculty of Medicine, Foundation for Medical Researches, Geneva University Hospitals, avenue de la Roseraie 64, 1211 Geneva 4, Switzerland
    • Pelli, Graziano. Division of Cardiology, Faculty of Medicine, Foundation for Medical Researches, Geneva University Hospitals, avenue de la Roseraie 64, 1211 Geneva 4, Switzerland
    • Kovari, Enikö. Department of Psychiatry, Geneva School of Medicine, Geneva, Switzerland
    • Pane, Bianca. Vascular and Endovascular Surgery Unit, Department of Surgery, San Martino Hospital, Genoa, Italy
    • Spinella, Giovanni. Vascular and Endovascular Surgery Unit, Department of Surgery, San Martino Hospital, Genoa, Italy
    • Pende, Aldo. First Medical Clinic, Laboratory of Phagocyte Physiopathology and Inflammation, Department of Internal Medicine, University of Genoa, Italy
    • Palombo, Domenico. Vascular and Endovascular Surgery Unit, Department of Surgery, San Martino Hospital, Genoa, Italy
    • Dallegri, Franco. First Medical Clinic, Laboratory of Phagocyte Physiopathology and Inflammation, Department of Internal Medicine, University of Genoa, Italy
    • Mach, François. Division of Cardiology, Faculty of Medicine, Foundation for Medical Researches, Geneva University Hospitals, avenue de la Roseraie 64, 1211 Geneva 4, Switzerland
    • Roux-Lombard, Pascale. Division of Laboratory Medicine, Department of Genetics and Laboratory Medicine, Geneva University Hospitals, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • European Heart Journal, 2011, vol. 32, no. 4, p. 412-421. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:298725
    Summary
    Aims Anti-Apolipoprotein A-1 auto-antibodies (anti-ApoA-1 IgG) represent an emerging prognostic cardiovascular marker in patients with myocardial infarction or autoimmune diseases associated with high cardiovascular risk. The potential relationship between anti-ApoA-1 IgG and plaque vulnerability remains elusive. Thus, we aimed to investigate the role of anti-ApoA-1 IgG in plaque vulnerability. Methods and results Potential relationship between anti-ApoA-1 IgG and features of cardiovascular vulnerability was explored both in vivo and in vitro. In vivo, we investigated anti-ApoA-1 IgG in patients with severe carotid stenosis (n = 102) and in ApoE−/− mice infused with polyclonal anti-ApoA-1 IgG. In vitro, anti-ApoA-1 IgG effects were assessed on human primary macrophages, monocytes, and neutrophils. Intraplaque collagen was decreased, while neutrophil and matrix metalloprotease (MMP)-9 content were increased in anti-ApoA-1 IgG-positive patients and anti-ApoA-1 IgG-treated mice when compared with corresponding controls. In mouse aortic roots (but not in abdominal aortas), treatment with anti-ApoA-1 IgG was associated with increased lesion size when compared with controls. In humans, serum anti-ApoA-1 IgG levels positively correlated with intraplaque macrophage, neutrophil, and MMP-9 content, and inversely with collagen. In vitro, anti-ApoA-1 IgG increased macrophage release of CCL2, CXCL8, and MMP-9, as well as neutrophil migration towards TNF-α or CXCL8. Conclusion These results suggest that anti-ApoA-1 IgG might be associated with increased atherosclerotic plaque vulnerability in humans and mice