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Hydrogen Sulfide Measurement by Headspace-gas Chromatography-mass Spectrometry (HS-GC-MS): Application to Gaseous Samples and Gas Dissolved in Muscle

Varlet, Vincent ; Giuliani, Nicole ; Palmiere, Cristian ; Maujean, Géraldine ; Augsburger, Marc

In: Journal of Analytical Toxicology, 2015, vol. 39, no. 1, p. 52-57

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    Titel
    • Hydrogen Sulfide Measurement by Headspace-gas Chromatography-mass Spectrometry (HS-GC-MS): Application to Gaseous Samples and Gas Dissolved in Muscle
    Autor
    • Varlet, Vincent. Forensic Toxicology and Chemistry Unit, University Centre of Legal Medicine Lausanne- Geneva CH-1011 Lausanne, Switzerland
    • Giuliani, Nicole. Forensic Toxicology and Chemistry Unit, University Centre of Legal Medicine Lausanne- Geneva CH-1011 Lausanne, Switzerland
    • Palmiere, Cristian. Forensic Medicine Unit, University Centre of Legal Medicine Lausanne- Geneva CH-1011 Lausanne, Switzerland
    • Maujean, Géraldine. Institut Universitaire de Médecine Légale, 12 Avenue Rockefeller, 69005 Lyon, France
    • Augsburger, Marc. Forensic Toxicology and Chemistry Unit, University Centre of Legal Medicine Lausanne- Geneva CH-1011 Lausanne, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Journal of Analytical Toxicology, 2015, vol. 39, no. 1, p. 52-57. Oxford University Press
    Andere elektronische Ausgabe
    Klassifikation
    • Gesundheit
    OAI-PMH-ID
    • oai:doc.rero.ch:297854
    Summary
    The aim of our study was to present a new headspace-gas chromatography-mass spectrometry (HS-GC-MS) method applicable to the routine determination of hydrogen sulfide (H2S) concentrations in biological and gaseous samples. The primary analytical drawback of the GC/MS methods for H2S measurement discussed in the literature was the absence of a specific H2S internal standard required to perform quantification. Although a deuterated hydrogen sulfide (D2S) standard is currently available, this standard is not often used because this standard is expensive and is only available in the gas phase. As an alternative approach, D2S can be generated in situ by reacting deuterated chloride with sodium sulfide; however, this technique can lead to low recovery yield and potential isotopic fractionation. Therefore, N2O was chosen for use as an internal standard. This method allows precise measurements of H2S concentrations in biological and gaseous samples. Therefore, a full validation using accuracy profile based on the β-expectation tolerance interval is presented. Finally, this method was applied to quantify H2S in an actual case of H2S fatal intoxication