High Frequency of Cytomegalovirus-Specific Cytotoxic T-Effector Cells in HLA-A*0201-Positive Subjects during Multiple Viral Coinfections

Jin, Xia ; Demoitie, Marie-Ange ; Donahoe, Sean M. ; Ogg, Graham S. ; Bonhoeffer, Sebastian ; Kakimoto, William M. ; Gillespie, Geraldine ; Moss, Paul A. ; Dyer, Wayne ; Kurilla, Michael G. ; Riddell, Stanley R. ; Downie, Jean ; Sullivan, John S. ; McMichael, Andrew J. ; Workman, Cassy ; Nixon, Douglas F.

In: The Journal of Infectious Diseases, 2000, vol. 181, no. 1, p. 165-175

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    Summary
    How the cellular immune response copes with diverse antigenic competition is poorly understood. Responses of virus-specific cytotoxic T lymphocytes (CTL) were examined longitudinally in an individual coinfected with human immunodeficiency virus type 1 (HIV-1), Epstein-Barr virus (EBV), and cytomegalovirus (CMV). CTL responses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201 tetrameric complexes folded with HIV-1, EBV, and CMV peptides. A predominance of CMV-pp65-speciflc CTL was found, with a much lower frequency of CTL to HIV-1 Gag and Pol and to EBV-BMLF1 and LMP2. The high frequency of CMV-speciflc CTL, compared with HIV-1- and EBV-specific CTL, was confirmed in an additional 16 HLA-A*0201-positive virus-coinfected subjects. Therefore, the human immune system can mount CTL responses to multiple viral antigens simultaneously, albeit with different strengths