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Podoplanin-positive cells are a hallmark of encapsulating peritoneal sclerosis

Braun, Niko ; Alscher, Dominik M. ; Fritz, Peter ; Edenhofer, Ilka ; Kimmel, Martin ; Gaspert, Ariana ; Reimold, Fabian ; Bode-Lesniewska, Beata ; Ziegler, Urs ; Biegger, Dagmar ; Wüthrich, Rudolf P. ; Segerer, Stephan

In: Nephrology Dialysis Transplantation, 2011, vol. 26, no. 3, p. 1033-1041

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    Titre
    • Podoplanin-positive cells are a hallmark of encapsulating peritoneal sclerosis
    Auteur
    • Braun, Niko. Department of Internal Medicine, Division of General Internal Medicine and Nephrology, Robert-Bosch-Hospital, Stuttgart, Germany
    • Alscher, Dominik M.. Department of Internal Medicine, Division of General Internal Medicine and Nephrology, Robert-Bosch-Hospital, Stuttgart, Germany
    • Fritz, Peter. Institute of Digital Medicine, Stuttgart, Germany
    • Edenhofer, Ilka. Division of Nephrology, University Hospital Zurich, Zurich, Switzerland
    • Kimmel, Martin. Department of Internal Medicine, Division of General Internal Medicine and Nephrology, Robert-Bosch-Hospital, Stuttgart, Germany
    • Gaspert, Ariana. Department of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland
    • Reimold, Fabian. Department of Internal Medicine, Division of General Internal Medicine and Nephrology, Robert-Bosch-Hospital, Stuttgart, Germany
    • Bode-Lesniewska, Beata. Department of Surgical Pathology, University Hospital Zurich, Zurich, Switzerland
    • Ziegler, Urs. Center of Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland
    • Biegger, Dagmar. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, Germany
    • Wüthrich, Rudolf P.. Division of Nephrology, University Hospital Zurich, Zurich, Switzerland
    • Segerer, Stephan. Division of Nephrology, University Hospital Zurich, Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Nephrology Dialysis Transplantation, 2011, vol. 26, no. 3, p. 1033-1041. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:296485
    Summary
    Background. Encapsulating peritoneal sclerosis (EPS) and simple peritoneal sclerosis are important complications of long-term peritoneal dialysis (PD). Podoplanin is expressed by mesothelial cells and lymphatic vessels, which are involved in inflammatory reactions in the peritoneal cavity. Methods. We studied 69 peritoneal biopsies from patients on PD (n = 16), patients with EPS (n = 18) and control biopsies taken at the time of hernia repair (n = 15) or appendectomy (n = 20). Immunohistochemistry was performed to localize podoplanin. Additionally, markers of endothelial cells, mesothelial cells, myofibroblasts (smooth muscle actin), proliferating cells, and double labelling for smooth muscle actin/podoplanin were used on selected biopsies. Results. Podoplanin was present on the endothelium of lymphatic vessels in the submesothelial fibrous tissue and on mesothelial cells. In patients on PD and in biopsies with appendicitis, the mesothelial cells demonstrated a cuboidal appearance and circumferential podoplanin staining, with gaps between the cells. The number of lymphatic vessels was variable, but prominent at sites of fibrosis. In patients with EPS, a diffuse infiltration of podoplanin-positive cells with a fibroblastic appearance was present in 15 out of 18 biopsies. This pattern was focally present in 3 out of 16 on PD and none in the 35 controls. The podoplanin-positive cells did not express the endothelial marker or the mesothelial marker (calretinin). Conclusions. EPS is characterized by a population of podoplanin and smooth muscle actin double-positive cells. Podoplanin might be a suitable morphological marker supporting the diagnosis and might be involved in the pathogenesis of EPS