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000029634 005__ 20130225122324.0
000029634 0248_ $$aoai:doc.rero.ch:20120815120637-BE$$ppostprint$$prero_explore$$zthesis_urn$$zpreprint$$zreport$$zthesis$$zbook$$zjournal$$zcdu16$$zcdu556$$zcdu1$$zdissertation$$zunine$$zcdu34
000029634 041__ $$aeng
000029634 080__ $$a556
000029634 100__ $$aYumiko, Abe$$uCentre for Hydrogeology, University of Neuchâtel, Switzerland
000029634 245__ $$9eng$$aEffect of molecule size on carbon isotope fractionation during biodegradation of chlorinated alkanes by Xanthobacter autotrophicus GJ10
000029634 520__ $$9eng$$aThe effect of the number of carbon and chlorine atoms on carbon isotope fractionation during dechlorination of chlorinated alkanes by <i>Xanthobacter autotrophicus</i> GJ10 was studied using pure culture and cell-free extract experiments. The magnitude of carbon isotope fractionation decreased with increasing carbon number. The decrease can be explained by an increasing probability that the heavy isotope is located at a non-reacting position for increasing molecule size. The isotope data were corrected for the number of carbons as well as the number of reactive sites to obtain reacting-site-specific values denoted as apparent kinetic isotope effect (AKIE). Even after the correction, the obtained AKIE values varied (on average 1.0608, 1.0477, 1.0616, and 1.0555 for 1,2-dichloroethane, chloropentane, 1,3-dichloropentane and chlorobutane, respectively). Cell-free extract experiments were carried out to evaluate the effect of transport across the cell membrane on the observed variability in the AKIE values, which revealed that variability still persisted. The study demonstrates that even after differences related to the carbon number and structure of the molecule are taken into account, there still remain differences in AKIE values even for compounds that are degraded by the same pure culture and an identical reaction mechanism.
000029634 695__ $$9eng$$abacterial cultures ; carbon-13 ; chlorinated alkanes ; degradation ; enzymes ; kinetic isotope effect ; molecule size
000029634 700__ $$aZopfi, Jakob$$uLaboratory of Microbiology, University of Neuchâtel, Switzerland
000029634 700__ $$aHunkeler, Daniel$$uCentre for Hydrogeology, University of Neuchâtel, Switzerland
000029634 773__ $$dTaylor & Francis$$g2009/45/1/18-26$$tIsotopes in Environmental and Health Studies
000029634 775__ $$gPublished Version$$ohttp://dx.doi.org/10.1080/10256010802522051
000029634 8564_ $$fAbe_Yumiko_Effect_of_molecule_size_on_carbon_isotope_20120815.pdf$$qapplication/pdf$$s647151$$uhttp://doc.rero.ch/record/29634/files/Abe_Yumiko_Effect_of_molecule_size_on_carbon_isotope_20120815.pdf$$yorder:1$$zTexte intégral
000029634 918__ $$aFaculté des sciences$$bRue Emile-Argand 11, 2007 Neuchâtel$$cHydrologie
000029634 919__ $$aUniversité de Neuchâtel$$bNeuchâtel$$ddoc.support@rero.ch
000029634 980__ $$aPOSTPRINT$$bUNINE$$fART_JOURNAL
000029634 990__ $$a20120815120637-BE