000029601 001__ 29601
000029601 005__ 20130225122324.0
000029601 0248_ $$aoai:doc.rero.ch:20120717094507-TD$$ppostprint$$prero_explore$$zthesis_urn$$zreport$$zthesis$$zcdu58$$zbook$$zjournal$$zcdu16$$zpreprint$$zcdu1$$zdissertation$$zunine$$zcdu34
000029601 041__ $$aeng
000029601 080__ $$a58
000029601 100__ $$aJunier, Pilar$$uLaboratory of Microbiology, Institute of Biology, University of Neuchâtel, Switzerland
000029601 245__ $$9eng$$aGenetic diversity of cyanobacterial communities in Lake Kinneret (Israel) using 16S rRNA gene, <i>psbA</i> and <i>ntcA</i> sequence analyses
000029601 269__ $$c2007
000029601 520__ $$9eng$$aThe genetic diversity of cyanobacterial communities was studied at various depths in Lake Kinneret (Israel). Denaturing gradient gel electrophoresis (DGGE) of specific 16S rRNA gene PCR products showed significant differences in the cyanobacterial community structure between epi- and hypolimnetic waters. Sequences of clone libraries prepared from 16S rRNA gene PCR products from epi- and hypolimnion revealed the presence of at least 11 different groups of cyanobacteria. Clones related to the unicellular cyanobacteria (Chroococcales and picocyanobacteria) dominated the clone libraries from both depths. New primers to amplify the gene coding for the photosystem II reaction centre (<i>psbA</i>) and the nitrogen regulator gene (<i>ntcA</i>) of cyanobacteria were developed and used for further characterization of the cyanobacterial communities from the lake. Sequences of <i>psbA</i> amplicons clustered with those from 2 different groups of marine <i>Synechococcus</i> and Chroococcales. Cloned <i>ntcA</i> amplicons from the lake were closely related and did not cluster with sequences from cultured cyanobacteria or other environmental sequences from this gene. All the molecular markers analyzed here showed similarity to sequences from some groups of cyanobacteria in the lake and those so far found in marine habitats.
000029601 695__ $$9eng$$a<i>Gloeocapsa</i> ; Picophytoplankton ; 16S rRNA gene ; <i>psbA</i> ; <i>ntcA</i>
000029601 700__ $$aWitzel, Karl-Paul$$uMax Planck Institute for Limnology, Plön, Germany
000029601 700__ $$aHadas, Ora$$uIsrael Oceanographic and Limnological Research, the Kinneret Limnological Laboratory, Migdal, Israel
000029601 773__ $$dInter-Research$$g2007/49/3/233-241$$tAquatic Microbial Ecology
000029601 775__ $$gPublished Version$$ohttp://dx.doi.org/10.3354/ame01161
000029601 8564_ $$fJunier_Pilar_-_Genetic_diversity_of_cyanobacterial_communities_in_Lake_INTER-RESEARCH_20120717.pdf$$qapplication/pdf$$s1362075$$uhttp://doc.rero.ch/record/29601/files/Junier_Pilar_-_Genetic_diversity_of_cyanobacterial_communities_in_Lake_INTER-RESEARCH_20120717.pdf$$yorder:1$$zTexte intégral
000029601 918__ $$aFaculté des sciences$$bRue Emile-Argand 11, 2007 Neuchâtel$$cInstitut de biologie
000029601 919__ $$aUniversité de Neuchâtel$$bNeuchâtel$$ddoc.support@rero.ch
000029601 980__ $$aPOSTPRINT$$bUNINE$$fART_JOURNAL
000029601 990__ $$a20120717094507-TD