Fulltext

Increased bioavailability of hesperetin-7-glucoside compared with hesperidin results in more efficient prevention of bone loss in adult ovariectomised rats

Habauzit, Véronique ; Nielsen, Inge-Lise ; Gil-Izquierdo, Angel ; Trzeciakiewicz, Anna ; Morand, Christine ; Chee, Winnie ; Barron, Denis ; Lebecque, Patrice ; Davicco, Marie-Jeanne ; Williamson, Gary ; Offord, Elizabeth ; Coxam, Véronique ; Horcajada, Marie-Noëlle

In: British Journal of Nutrition, 2009, vol. 102, no. 7, p. 976-984

Ajouter à la liste personnelle
    Titre
    • Increased bioavailability of hesperetin-7-glucoside compared with hesperidin results in more efficient prevention of bone loss in adult ovariectomised rats
    Auteur
    • Habauzit, Véronique. Human Nutrition Unit, UMR 1019, ASM, INRA, Clermont-Ferrand/Theix, St-Genès Champanelle F-63122, France
    • Nielsen, Inge-Lise. Nestlé Research Center, Vers Chez Les Blanc, 1000 Lausanne 26, Switzerland
    • Gil-Izquierdo, Angel. Departamento de Ciencia y Tecnología de Alimentos, CEBAS - CSIC, 30100 Espinardo, Murcia, Spain
    • Trzeciakiewicz, Anna. Human Nutrition Unit, UMR 1019, ASM, INRA, Clermont-Ferrand/Theix, St-Genès Champanelle F-63122, France
    • Morand, Christine. Human Nutrition Unit, UMR 1019, ASM, INRA, Clermont-Ferrand/Theix, St-Genès Champanelle F-63122, France
    • Chee, Winnie. Nestlé Research Center, Vers Chez Les Blanc, 1000 Lausanne 26, Switzerland
    • Barron, Denis. Nestlé Research Center, Vers Chez Les Blanc, 1000 Lausanne 26, Switzerland
    • Lebecque, Patrice. Human Nutrition Unit, UMR 1019, ASM, INRA, Clermont-Ferrand/Theix, St-Genès Champanelle F-63122, France
    • Davicco, Marie-Jeanne. Human Nutrition Unit, UMR 1019, ASM, INRA, Clermont-Ferrand/Theix, St-Genès Champanelle F-63122, France
    • Williamson, Gary. Nestlé Research Center, Vers Chez Les Blanc, 1000 Lausanne 26, Switzerland
    • Offord, Elizabeth. Nestlé Research Center, Vers Chez Les Blanc, 1000 Lausanne 26, Switzerland
    • Coxam, Véronique. Human Nutrition Unit, UMR 1019, ASM, INRA, Clermont-Ferrand/Theix, St-Genès Champanelle F-63122, France
    • Horcajada, Marie-Noëlle. Human Nutrition Unit, UMR 1019, ASM, INRA, Clermont-Ferrand/Theix, St-Genès Champanelle F-63122, France
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • British Journal of Nutrition, 2009, vol. 102, no. 7, p. 976-984. Cambridge University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:294960
    Summary
    Hesperidin (Hp), a citrus flavonoid predominantly found in oranges, shows bone-sparing effects in ovariectomised (OVX) animals. In human subjects, the bioavailability of Hp can be improved by the removal of the rhamnose group to yield hesperetin-7-glucoside (H-7-glc). The aim of the present work was to test whether H-7-glc was more bioavailable and therefore more effective than Hp in the prevention of bone loss in the OVX rat. Adult 6-month-old female Wistar rats were sham operated or OVX, then pair fed for 90d a casein-based diet supplemented or not with freeze-dried orange juice enriched with Hp or H-7-glc at two dose equivalents of the hesperetin aglycone (0·25 and 0·5%). In the rats fed 0·5%, a reduction in OVX-induced bone loss was observed regarding total bone mineral density (BMD):+7·0% in OVX rats treated with Hp (HpOVX) and +6·6% in OVX rats treated with H-7-glc (H-7-glcOVX) v. OVX controls (P<0·05). In the rats fed 0·25% hesperetin equivalents, the H-7-glcOVX group showed a 6·6% improvement in total femoral BMD v. the OVX controls (P<0·05), whereas the Hp diet had no effect at this dose. The BMD of rats fed 0·25% H-7-glc was equal to that of those given 0·5% Hp, but was not further increased at 0·5% H-7-glc. Plasma hesperetin levels and relative urinary excretion were significantly enhanced in the H-7-glc v. Hp groups, and the metabolite profile showed the absence of eriodictyol metabolites and increased levels of hesperetin sulphates. Taken together, improved bioavailability of H-7-glc may explain the more efficient bone protection of this compound