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TGFβ receptor II gene deletion in leucocytes prevents cerebral vasculitis in bacterial meningitis

Malipiero, Ursula ; Koedel, Uwe ; Pfister, Hans-Walter ; Levéen, Per ; Bürki, Kurt ; Reith, Walter ; Fontana, Adriano

In: Brain, 2006, vol. 129, no. 9, p. 2404-2415

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    Titre
    • TGFβ receptor II gene deletion in leucocytes prevents cerebral vasculitis in bacterial meningitis
    Auteur
    • Malipiero, Ursula. Clinical Immunology, University Hospital Zurich Zurich, Switzerland
    • Koedel, Uwe. Department of Neurology, Klinikum Grosshadern, Ludwig Maximilians University Munich, Germany
    • Pfister, Hans-Walter. Department of Neurology, Klinikum Grosshadern, Ludwig Maximilians University Munich, Germany
    • Levéen, Per. Department of Molecular Medicine and Gene Therapy, Lund University Lund, Sweden
    • Bürki, Kurt. Institute of Laboratory Animal Science, University of Zurich Zurich, Switzerland
    • Reith, Walter. Department of Pathology and Immunology, Centre Médicale Universitaire (CMU) Geneva, Switzerland
    • Fontana, Adriano. Clinical Immunology, University Hospital Zurich Zurich, Switzerland
    Type de document
    • Postprint
    Langue
    • Inglese
    Publié dans
    • Brain, 2006, vol. 129, no. 9, p. 2404-2415. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:294873
    Summary
    In bacterial meningitis, chemokines lead to recruitment of polymorphonuclear leucocytes (PMN) into the CNS. At the site of infection in the subarachnoid space, PMN release reactive oxygen species, reactive nitrogen intermediates (RNI) and interleukin-1β (IL-1β). Although these immune factors assist in clearance of bacteria, they also result in neuronal injury associated with meningitis. Transforming growth factor beta (TGFβ) is a potent deactivator of PMN and macrophages since TGFβ suppresses the production of ROI, RNI and IL-1. Here, we report that the deletion of the TGFβ receptor II gene in PMN enhances PMN recruitment into the CNS of mice with Streptococcus pneumoniae meningitis. This was associated with more efficient clearance of bacteria, and almost complete prevention of intracerebral necrotizing vasculitis. Differences in PMN in the CNS of infected control mice and mice lacking TGFβ receptor II were not explained by altered expression of chemokines acting on PMN. Instead, TGFβ was found to impair the expression of l (leucocyte)-selectin on PMN from control mice but not from mice lacking TGFβ receptor II. l-Selectin is known to be essential for PMN recruitment in bacterial meningitis. We conclude that defective TGFβ signalling in PMN is beneficial in bacterial meningitis by ameliorating migration of PMN and bacterial clearance