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Lotus japonicus Nodulates and Fixes Nitrogen with the Broad Host Range Rhizobium sp. NGR234

Hussain, A.K.M. Anwar ; Jiang, Qunyi ; Broughton, William J. ; Gresshoff, Peter M.

In: Plant and Cell Physiology, 1999, vol. 40, no. 8, p. 894-899

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    Titre
    • Lotus japonicus Nodulates and Fixes Nitrogen with the Broad Host Range Rhizobium sp. NGR234
    Auteur
    • Hussain, A.K.M. Anwar. Plant Molecular Genetics and Center for Legume Research, The University of Tennessee Knoxville, TN 37901-1071, U.S.A.
    • Jiang, Qunyi. Plant Molecular Genetics and Center for Legume Research, The University of Tennessee Knoxville, TN 37901-1071, U.S.A.
    • Broughton, William J.. Laboratoire de Biologie Moleculaire des Planles Superieures, University of Geneva CH-1292 Chambesy, Switzerland
    • Gresshoff, Peter M.. Plant Molecular Genetics and Center for Legume Research, The University of Tennessee Knoxville, TN 37901-1071, U.S.A.
    Type de document
    • Postprint
    Identifiant OAI-PMH
    • oai:doc.rero.ch:294451
    Summary
    Lotus japonicus possesses major advantages as a model legume for the study of plant-microbe interactions. The relative absence of genetic information on its normal microbial partner (i.e., Mesorhizobium loti) could limit its utility in research. Here we show for the first time that the broad host range Rhizobium strain NGR234 nodulates and fixes nitrogen in symbiosis with Lotus japonicus ecotypes "Gifu” and "Funakura”. We demonstrate that bacterial mutants deficient in nodulation or nitrogen fixation possess the expected phenotype with L.japonicus. Nodulation of L.japonicus was sensitive to nitrate. Vermiculite was an efficient synthetic growth substrate, allowing axenic growth in Magenta jars. The genetic analysis of the Lotus japonicus-Mesorhizobium interaction should be accelerated through the use of this well-defined microsymbiont