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Imaging of Vascular Smooth Muscle Cells with Soft X-Ray Spectromicroscopy

Sedlmair, Julia ; Gleber, Sophie-Charlotte ; Mert, Semra Öztürk ; Bertilson, Michael ; von Hofsten, Olov ; Thieme, Jürgen ; Pfohl, Thomas

In: Microscopy and Microanalysis, 2011, vol. 17, no. 6, p. 991-1001

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    Titre
    • Imaging of Vascular Smooth Muscle Cells with Soft X-Ray Spectromicroscopy
    Auteur
    • Sedlmair, Julia. Institute for X-Ray Physics, Georg-August-University Göttingen, Friedrich-Hund-Pl. 1, D-37077 Göttingen, Germany
    • Gleber, Sophie-Charlotte. Argonne National Laboratory, APS, 9700 S. Cass Avenue, Building 401, Argonne, IL 60439-4837, USA
    • Mert, Semra Öztürk. Max-Planck-Institute for Dynamics and Self-Organization, Bunsenstr. 10, D-37073, Göttingen, Germany
    • Bertilson, Michael. Biomedical and X-Ray Physics, Department of Applied Physics, Royal Institute of Technology, AlbaNova, SE-106 91 Stockholm, Sweden
    • von Hofsten, Olov. Biomedical and X-Ray Physics, Department of Applied Physics, Royal Institute of Technology, AlbaNova, SE-106 91 Stockholm, Sweden
    • Thieme, Jürgen. Brookhaven National Laboratory, NSLS II, Building 817, Upton, NY 11973, USA
    • Pfohl, Thomas. Max-Planck-Institute for Dynamics and Self-Organization, Bunsenstr. 10, D-37073, Göttingen, Germany
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Microscopy and Microanalysis, 2011, vol. 17, no. 6, p. 991-1001. Cambridge University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:293932
    Summary
    Using X-ray microscopy and spectromicroscopy, vascular smooth muscle cells (VSMCs) were imaged, prepared without using additional embedding material or staining, but by applying simple, noncryo fixation techniques. The cells were imaged with a compact source transmission X-ray microscope and a scanning transmission X-ray microscope (STXM). With the STXM, spectromicroscopy was performed at the C K-edge and the Ca LIII,II-edges. VSMCs were chosen because of their high amount of actin stress fibers, so that the actin cytoskeleton should be visible. Other parts of the cell, such as the nucleus and organelles, were also identified from the micrographs. Both in the spectra and the images, the effects of the different preparation procedures were observable. Furthermore, Ca hotspots were detected and their density is determined