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Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

Osaka, Masaaki ; Matsuda, Tomoki ; Sakazono, Satomi ; Masuko-Suzuki, Hiromi ; Maeda, Shunsuke ; Sewaki, Misato ; Sone, Mikako ; Takahashi, Hirokazu ; Nakazono, Mikio ; Iwano, Megumi ; Takayama, Seiji ; Shimizu, Kentaro K. ; Yano, Kentaro ; Lim, Yong Pyo ; Suzuki, Go ; Suwabe, Keita ; Watanabe, Masao

In: Plant and Cell Physiology, 2013, vol. 54, no. 11, p. 1894-1906

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    Titel
    • Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing
    Autor
    • Osaka, Masaaki. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan
    • Matsuda, Tomoki. Graduate School of Bioresources, Mie University, Tsu, 514-8507 Japan
    • Sakazono, Satomi. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan
    • Masuko-Suzuki, Hiromi. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan
    • Maeda, Shunsuke. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan
    • Sewaki, Misato. Graduate School of Bioresources, Mie University, Tsu, 514-8507 Japan
    • Sone, Mikako. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan
    • Takahashi, Hirokazu. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan
    • Nakazono, Mikio. Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan
    • Iwano, Megumi. Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0192 Japan
    • Takayama, Seiji. Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0192 Japan
    • Shimizu, Kentaro K.. Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
    • Yano, Kentaro. Faculty of Agriculture, Meiji University, Kawasaki, 214-8571 Japan
    • Lim, Yong Pyo. Department of Horticulture, Chungnam National University, Daejeon 305-764, Republic of Korea
    • Suzuki, Go. Division of Natural Science, Osaka Kyoiku University, Kashiwara 582-8582, Japan
    • Suwabe, Keita. Graduate School of Bioresources, Mie University, Tsu, 514-8507 Japan
    • Watanabe, Masao. Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • Plant and Cell Physiology, 2013, vol. 54, no. 11, p. 1894-1906. Oxford University Press
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:293017
    Summary
    Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms