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Phytophthora polonica, a new species isolated from declining Alnus glutinosa stands in Poland

Belbahri, Lassaad ; Moralejo, Eduardo ; Calmin, Gautier ; Oszako, Tomasz ; García, Jose A. ; Descals, Enrique ; Lefort, Francois

In: FEMS Microbiology Letters, 2006, vol. 261, no. 2, p. 165-174

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    Title
    • Phytophthora polonica, a new species isolated from declining Alnus glutinosa stands in Poland
    Author
    • Belbahri, Lassaad. Laboratory of Applied Genetics, School of Engineering of Lullier, University of Applied Sciences of Western Switzerland, Jussy, Switzerland
    • Moralejo, Eduardo. Instituto Mediterráneo de Estudios Avanzados, IMEDEA (CSIC-UIB), Esporles, Balearic Islands, Spain
    • Calmin, Gautier. Laboratory of Applied Genetics, School of Engineering of Lullier, University of Applied Sciences of Western Switzerland, Jussy, Switzerland
    • Oszako, Tomasz. Department of Forest Phytopathology, Forest Research Institute, Raszyn, Poland
    • García, Jose A.. Instituto Mediterráneo de Estudios Avanzados, IMEDEA (CSIC-UIB), Esporles, Balearic Islands, Spain
    • Descals, Enrique. Instituto Mediterráneo de Estudios Avanzados, IMEDEA (CSIC-UIB), Esporles, Balearic Islands, Spain
    • Lefort, Francois. Laboratory of Applied Genetics, School of Engineering of Lullier, University of Applied Sciences of Western Switzerland, Jussy, Switzerland
    Document Type
    • Postprint
    OAI-PMH Identifier
    • oai:doc.rero.ch:292166
    Summary
    In a survey of Phytophthora associated with alder decline in Poland, several isolates of a homothallic Phytophthora spet al, which could not be assigned to other taxa including Phytophthora alni subspecies, were consistently recovered from rhizosphere soil samples. Their morphology and pathogenicity, as well as sequence data for three nuclear regions (internal transcribed spacer rDNA, elongation factor-1α and β-tubulin) and a coding mitochondrial DNA region (nadh1), were examined. The new Phytophthora species is characterized by the moderate to slow growth rate of its colony in carrot agar at 20°C, high optimal (c. 30°C) and maximum (c. 38°C) growth temperatures, formation of catenulate, often lateral, hyphal swellings, large chlamydospores in agar media and in soil extract, persistent, ovoid to ellipsoid nonpapillate sporangia and large oogonia with paragynous and sometimes amphigynous antheridia. Phytophthora polonica was slightly pathogenic to alder twigs and not pathogenic to trunks of several tree species. In a phylogenetic analysis using either Bayesian inference or maximum likelihood methods, P. polonica falls in clade 8 ‘sensu Kroon (2004)' of Phytophthora