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Rearrangement and expression of χ light chain genes can occur without μ heavy chain expression during differentiation of pre-B cells

Grawunder, Ulf ; Haasner, Dirk ; Melchers, Fritz ; Rolink, Antonius

In: International Immunology, 1993, vol. 5, no. 12, p. 1609-1618

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    Titre
    • Rearrangement and expression of χ light chain genes can occur without μ heavy chain expression during differentiation of pre-B cells
    Auteur
    • Grawunder, Ulf. Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland
    • Haasner, Dirk. Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland
    • Melchers, Fritz. Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland
    • Rolink, Antonius. Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • International Immunology, 1993, vol. 5, no. 12, p. 1609-1618. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:290556
    Summary
    The kinetics of χ light (χL) chain gene rearrangement and expression on mRNA and protein level has been studied with four stromal cell/IL-7 reactive, long-term in vitro proliferating pre-B cell lines and clones, two from fetal liver of normal mice and two from fetal liver of EμH-bcl-2 transgenic (bcl-2-tg) mice. These pre-B cell lines and clones are DJH-rearranged on both H chain alleles. Two of the clones harbor H chain rearrangements which do not allow the expression of VHDJH rearranged H chain genes as μH chain proteins. Upon removal of IL-7 from the pre-B cell cultures all four cell lines rearrange VH-DJH and VL-JL gene segments, loose the surface expression of c-kit, CD43, and surrogate light chain, as well as the capacity to be clonable on stromal cells in the presence of IL-7. Pre-B cells from normal mice die by apoptosis during differentiation, while those from bcl-2-tg mice do not. All four lines and clones express comparable levels of mRNA for μH and μL chains with the same time kinetics during 3 days of differentiation. However, only two of the four pre-B cell lines and clones express μH chain protein, whereas all four pre-B cell lines and clones express μL chain protein at comparable levels between 2×105 and 1.40×106 μL chain molecules per cell. These results suggest that μH chain expression is not mandatory for rearrangement and normal expression of μL chain genes when pre-B cells differentiate to B cells