Fulltext

Injectable living marrow stromal cell-based autologous tissue engineered heart valves: first experiences with a one-step intervention in primates

Weber, Benedikt ; Scherman, Jacques ; Emmert, Maximilian Y. ; Gruenenfelder, Juerg ; Verbeek, Renier ; Bracher, Mona ; Black, Melanie ; Kortsmit, Jeroen ; Franz, Thomas ; Schoenauer, Roman ; Baumgartner, Laura ; Brokopp, Chad ; Agarkova, Irina ; Wolint, Petra ; Zund, Gregor ; Falk, Volkmar ; Zilla, Peter ; Hoerstrup, Simon P.

In: European Heart Journal, 2011, vol. 32, no. 22, p. 2830-2840

Zum persönliche Liste hinzufügen
    Titel
    • Injectable living marrow stromal cell-based autologous tissue engineered heart valves: first experiences with a one-step intervention in primates
    Autor
    • Weber, Benedikt. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    • Scherman, Jacques. Chris Barnard Division of Cardiothoracic Surgery, Groote Schuur Hospital, University of Cape Town, Cape Town, South Africa
    • Emmert, Maximilian Y.. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    • Gruenenfelder, Juerg. Department of Surgical Research, University Hospital of Zurich, Zurich, Switzerland
    • Verbeek, Renier. Cardiovascular Research Unit, Cape Heart Centre, University of Cape Town, Cape Town, South Africa
    • Bracher, Mona. Cardiovascular Research Unit, Cape Heart Centre, University of Cape Town, Cape Town, South Africa
    • Black, Melanie. Cardiovascular Research Unit, Cape Heart Centre, University of Cape Town, Cape Town, South Africa
    • Kortsmit, Jeroen. Cardiovascular Research Unit, Cape Heart Centre, University of Cape Town, Cape Town, South Africa
    • Franz, Thomas. Cardiovascular Research Unit, Cape Heart Centre, University of Cape Town, Cape Town, South Africa
    • Schoenauer, Roman. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    • Baumgartner, Laura. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    • Brokopp, Chad. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    • Agarkova, Irina. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    • Wolint, Petra. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    • Zund, Gregor. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    • Falk, Volkmar. Clinic for Cardiovascular Surgery, University Hospital of Zurich, Raemistrasse 100, 8091 CH-Zürich, Switzerland
    • Zilla, Peter. Chris Barnard Division of Cardiothoracic Surgery, Groote Schuur Hospital, University of Cape Town, Cape Town, South Africa
    • Hoerstrup, Simon P.. Swiss Center for Regenerative Medicine, Zurich, Switzerland
    Dokumententyp
    • Postprint
    Sprache
    • Englisch
    Veröffentlicht in
    • European Heart Journal, 2011, vol. 32, no. 22, p. 2830-2840. Oxford University Press
    Andere elektronische Ausgabe
    OAI-PMH-ID
    • oai:doc.rero.ch:290402
    Summary
    Aims A living heart valve with regeneration capacity based on autologous cells and minimally invasive implantation technology would represent a substantial improvement upon contemporary heart valve prostheses. This study investigates the feasibility of injectable, marrow stromal cell-based, autologous, living tissue engineered heart valves (TEHV) generated and implanted in a one-step intervention in non-human primates. Methods and results Trileaflet heart valves were fabricated from non-woven biodegradable synthetic composite scaffolds and integrated into self-expanding nitinol stents. During the same intervention autologous bone marrow-derived mononuclear cells were harvested, seeded onto the scaffold matrix, and implanted transapically as pulmonary valve replacements into non-human primates (n = 6). The transapical implantations were successful in all animals and the overall procedure time from cell harvest to TEHV implantation was 118 ± 17 min. In vivo functionality assessed by echocardiography revealed preserved valvular structures and adequate functionality up to 4 weeks post implantation. Substantial cellular remodelling and in-growth into the scaffold materials resulted in layered, endothelialized tissues as visualized by histology and immunohistochemistry. Biomechanical analysis showed non-linear stress-strain curves of the leaflets, indicating replacement of the initial biodegradable matrix by living tissue. Conclusion Here, we provide a novel concept demonstrating that heart valve tissue engineering based on a minimally invasive technique for both cell harvest and valve delivery as a one-step intervention is feasible in non-human primates. This innovative approach may overcome the limitations of contemporary surgical and interventional bioprosthetic heart valve prostheses