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Antigens of a Human Breast Carcinoma Cell Line (BT 20). I. Synthesis of Serum Proteins, Membrane-Associated Antigens, and Oncofetal-Associated Antigens

Hurlimann, J. ; Dayal, R.

In: Journal of the National Cancer Institute, 1978, vol. 61, no. 3, p. 677-685

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    Titre
    • Antigens of a Human Breast Carcinoma Cell Line (BT 20). I. Synthesis of Serum Proteins, Membrane-Associated Antigens, and Oncofetal-Associated Antigens
    Auteur
    • Hurlimann, J.. Department of Pathology, University of Lausanne, Medical School, 1011 Lausanne, Switzerland; and Department of Pathology, Hôpital Cantonal Universitaire, CH-1011 Lausanne, Switzerland.
    • Dayal, R.. Department of Pathology, University of Lausanne, Medical School, 1011 Lausanne, Switzerland; and Department of Pathology, Hôpital Cantonal Universitaire, CH-1011 Lausanne, Switzerland.
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Journal of the National Cancer Institute, 1978, vol. 61, no. 3, p. 677-685. Oxford University Press
    Autre version électronique
    Classification
    • Santé
    Identifiant OAI-PMH
    • oai:doc.rero.ch:289696
    Summary
    The synthesis of various products by a human mammary cell line (BT 20) was studied by incorporation of 14Clabeled amino acids, choline, glucosamine, or galactosamine into nondialyzable materials. These products had molecular weights ranging from less than 12,300 daltons to more than 200,000 daltons. They were analyzed by immunoelectrophoresis and double diffusion in agar. Among the synthesized products, the following proteins were identified: β2-glycoprotein I, α2HS-glycoprotein, α2-lipoprotein, actin, β2-microglobulin, carcinoembryonic antigen, three oncofetal-associated antigens, and various erythrocyte membrane-associated antigens (namely, glycophorin). Synthesis of milk proteins was not detectable. Only the protein moiety of the glycophorin molecule seemed to be synthesized. The β2-microglobulin was synthesized in an unbound state as well as bound to a glycoprotein whose relationship with the transplantation or tumor antigens must be determined. The three oncofetal-associated antigens were also synthesized in vitro by human fetal tissues and neoplastic and dysplastic human mammary tissues