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Bioactivation of N-nitrosomethylbenzylamine and N-nitrosomethyl-amylamine in oesophageal papillomas

Dirsch, O.R. ; Koenigsmann, M. ; Ludeke, B.I. ; Scherer, E. ; Kleihues, P.

In: Carcinogenesis, 1990, vol. 11, no. 9, p. 1583-1586

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    Titre
    • Bioactivation of N-nitrosomethylbenzylamine and N-nitrosomethyl-amylamine in oesophageal papillomas
    Auteur
    • Dirsch, O.R.. Laboratory of Neuropathology, Institute of Pathology University of Zürich, CH-8091 Zürich, Switzerland, Plesmanlaan 121, NL-1066CX Amsterdam, The Netherlands
    • Koenigsmann, M.. Laboratory of Neuropathology, Institute of Pathology University of Zürich, CH-8091 Zürich, Switzerland, Plesmanlaan 121, NL-1066CX Amsterdam, The Netherlands
    • Ludeke, B.I.. Laboratory of Neuropathology, Institute of Pathology University of Zürich, CH-8091 Zürich, Switzerland, Plesmanlaan 121, NL-1066CX Amsterdam, The Netherlands
    • Scherer, E.. Division of Chemical Carcinogenesis, The Netherlands Cancer Institute (Antoni van Leeuwenhoek Huis) Plesmanlaan 121, NL-1066CX Amsterdam, The Netherlands
    • Kleihues, P.. Laboratory of Neuropathology, Institute of Pathology University of Zürich, CH-8091 Zürich, Switzerland, Plesmanlaan 121, NL-1066CX Amsterdam, The Netherlands
    Type de document
    • Postprint
    Langue
    • Anglais
    Publié dans
    • Carcinogenesis, 1990, vol. 11, no. 9, p. 1583-1586. Oxford University Press
    Autre version électronique
    Identifiant OAI-PMH
    • oai:doc.rero.ch:289545
    Summary
    Oesophageal papillomas were induced in male F344 rats by continuous exposure to N-nitrosomethylbeazylamine (NMBZA) and N-nitrosomethyl(2-methylbutyl)ainine in the drinking water at concentrations of 10 and 19.5 p.p.m. respectively. After 81-141 days animals received a single i.p. chasing dose of NMIBZA (0.1 mmol/kg), [14C-methyl]NMBZA or N-nitroso[14C-methyl]amylamine and were killed 6 h later. Induced papillomas (3-9 per animal) were analysed by autoradlography and by immunohistochemistry using a polyclonal antibody to O6-methyldeoxyguanosine Both techniques revealed the presence of high levels of alkylation products in all papillomas investigated. Immunohistochemical staining of O6-methyldeoxyguanosine was largely restricted to nudei of the basal layer and of epithelial cells with incipient keratinization. These findings demonstrate that NMBZA and N-nitrosomethylamylamine and probably related methyl alkylnitrosamines are effectively bioactivated in premalignant lesions, indicating that during chronic exposure papifiomas can acquire additional mutations that are likely to play a major role in tumour progression