Rapid Aminoglycoside NP test for rapid detection of multiple aminoglycoside resistance in Enterobacteriaceae
- Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, France - INSERM European Unit (LEA, IAME, Paris), Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Institute of Microbiology, University Hospital Centre and University of Lausanne, Switzerland
- Jayol, Aurélie Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, France - INSERM European Unit (LEA, IAME, Paris), Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Laboratoire de Bactériologie, CHU de Bordeaux, France - CNRS UMR5234 Microbiologie Fondamentale et Pathogénicité, University of Bordeaux, France
- Dobias, Jan Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, France
- Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, France
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18.01.2017
Published in:
- Journal of Clinical Microbiology. - 2017, p. JCM.02107-16
English
The Rapid Aminoglycoside NP (Nordmann/Poirel) test was developed to rapidly identify multiple aminoglycoside (AG) resistance in Enterobacteriaceae. It is based on the detection of the glucose metabolism related to enterobacterial growth in presence of a defined concentration of amikacin plus gentamicin. Formation of acid metabolites was evidenced by a color change (orange to yellow) of the red phenol pH indicator. The Rapid Aminoglycoside NP test was evaluated by using bacterial colonies of 18 AG- resistant isolates producing 16S rRNA methylases, 20 AG-resistant isolates expressing AG-modifying enzymes (acetyl, adenyl and phospho transferases), and 10 isolates susceptible to AG. Its sensitivity and specificity were 100% and 97% respectively, as compared to the broth dilution method taken as the gold standard for determining aminoglycoside resistance. The test is inexpensive, rapid (<2 hours), and implementable worldwide.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 287915
- DOI 10.1128/JCM.02107-16
- Persistent URL
- https://folia.unifr.ch/unifr/documents/305303
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