Ultrathin ceramic membranes as scaffolds for functional cell coculture models on a biomimetic scale
- Jud, Corinne Adolphe Merkle Institute, University of Fribourg, Switzerland - Agroscope, Institute for Livestock Sciences ILS, Posieux, Switzerland
- Ahmed, Sher CSEM SA, Neuchâtel, Switzerland
- Müller, Loretta University Children's Hospital Basel, Switzerland
- Kinnear, Calum Adolphe Merkle Institute, University of Fribourg, Switzerland
- Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
- Umehara, Yuki Adolphe Merkle Institute, University of Fribourg, Switzerland
- Frey, Sabine Adolphe Merkle Institute, University of Fribourg, Switzerland
- Liley, Martha CSEM SA, Neuchâtel, Switzerland
- Angeloni, Silvia CSEM SA, Neuchâtel, Switzerland
- Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
- Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
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01.12.2015
Published in:
- BioResearch Open Access. - 2015, vol. 4, no. 1, p. 457–468
English
Epithelial tissue serves as an interface between biological compartments. Many in vitro epithelial cell models have been developed as an alternative to animal experiments to answer a range of research questions. These in vitro models are grown on permeable two-chamber systems; however, commercially available, polymer-based cell culture inserts are around 10 μm thick. Since the basement membrane found in biological systems is usually less than 1 μm thick, the 10-fold thickness of cell culture inserts is a major limitation in the establishment of realistic models. In this work, an alternative insert, accommodating an ultrathin ceramic membrane with a thickness of only 500 nm (i.e., the Silicon nitride Microporous Permeable Insert [SIMPLI]-well), was produced and used to refine an established human alveolar barrier coculture model by both replacing the conventional inserts with the SIMPLI-well and completing it with endothelial cells. The structural–functional relationship of the model was evaluated, including the translocation of gold nanoparticles across the barrier, revealing a higher translocation if compared to corresponding polyethylene terephthalate (PET) membranes. This study demonstrates the power of the SIMPLI-well system as a scaffold for epithelial tissue cell models on a truly biomimetic scale, allowing construction of more functionally accurate models of human biological barriers.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Chimie
- Language
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- English
- Classification
- Chemistry
- License
- License undefined
- Identifiers
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- RERO DOC 258334
- DOI 10.1089/biores.2015.0037
- Persistent URL
- https://folia.unifr.ch/unifr/documents/304771
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