Faculté des sciences

The genome of the Gram-positive metal- and sulfate-reducing bacterium Desulfotomaculum reducens strain MI-1

Junier, Pilar ; Junier, Thomas ; Podell, Sheila ; Sims, David R ; Detter, John C ; Lykidis, Athanasios ; Han, Cliff S ; Wigginton, Nicholas S ; Gaasterland, Terry ; Bernier-Latmani, Rizlan

In: Environmental Microbiology, 2010, vol. 12, no. 10, p. 2738–2754

Spore-forming, Gram-positive sulfate-reducing bacteria (SRB) represent a group of SRB that dominates the deep subsurface as well as niches in which resistance to oxygen and dessication is an advantage. Desulfotomaculum reducens; strain MI-1 is one of the few cultured representatives of that group with a complete genome sequence available. The metabolic versatility of this organism is... More

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    Summary
    Spore-forming, Gram-positive sulfate-reducing bacteria (SRB) represent a group of SRB that dominates the deep subsurface as well as niches in which resistance to oxygen and dessication is an advantage. Desulfotomaculum reducens; strain MI-1 is one of the few cultured representatives of that group with a complete genome sequence available. The metabolic versatility of this organism is reflected in the presence of genes encoding for the oxidation of various electron donors, including three- and four-carbon fatty acids and alcohols. Synteny in genes involved in sulfate reduction across all four sequenced Gram-positive SRB suggests a distinct sulfate-reduction mechanism for this group of bacteria. Based on the genomic information obtained for sulfate reduction in D. reducens;, the transfer of electrons to the sulfite and APS reductases is proposed to take place via the quinone pool and heterodisulfide reductases respectively. In addition, both H2-evolving and H2-consuming cytoplasmic hydrogenases were identified in the genome, pointing to potential cytoplasmic H2 cycling in the bacterium. The mechanism of metal reduction remains unknown.