Faculté des sciences

CarbAcineto NP test for rapid detection of carbapenemase-producers in Acinetobacter spp.

Dortet, Laurent ; Poirel, Laurent ; Errera, Caroline ; Nordmann, Patrice

In: Journal of Clinical Microbiology, 2014, p. JCM.00594–14

Multidrug-resistant Acinetobacter baumannii isolates, and particularly carbapenemase-producing isolates, are increasingly reported worldwide. The biochemically-based Carba NP test, extensively validated for detection of carbapenemase producers among Enterobacteriaceae and Pseudomonas spp., has been modified for the detection of carbapenemase production in Acinetobacter spp. A collection of 151... More

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    Summary
    Multidrug-resistant Acinetobacter baumannii isolates, and particularly carbapenemase-producing isolates, are increasingly reported worldwide. The biochemically-based Carba NP test, extensively validated for detection of carbapenemase producers among Enterobacteriaceae and Pseudomonas spp., has been modified for the detection of carbapenemase production in Acinetobacter spp. A collection of 151 carbapenemase-producing and 69 non-carbapenemase producing Acinetobacter spp. using the Carba NP test and a modified Carba NP protocol (CarbAcineto NP test) set up in this study. The CarbAcineto NP test requires modified lysis conditions and an increased bacterial inoculum as compared to the original Carba NP test. The Carba NP test detects metallo-β-lactamase producers but failed to detect production of other carbapenemase types among Acinetobacter spp. By contrast, the newly-designed CarbAcineto NP test, which is rapid and reproducible, detects all types of carbapenemases, with a sensitivity of 94.7% and a specificity of 100%. This cost-effective technique offers a reliable and affordable technique for identification of carbapenemase production in Acinetobacter spp., which is a marker of multidrug resistance in those species. Its use will facilitate their recognition and prevent their spread.