Faculté des sciences et de médecine

Dissemination of carbapenemase-producing Enterobacteriaceae in France, 2012

Dortet, Laurent ; Cuzon, Gaelle ; Nordmann, Patrice

In: Journal of Antimicrobial Chemotherapy, 2014, vol. 69, no. 3, p. 623-627

Objectives: Carbapenem-resistant Enterobacteriaceae isolates (n = 1485) were received at the French Associated National Reference Center for Antibiotic Resistance in 2012 and were characterized for their mechanism of resistance to carbapenems.Methods: Carbapenemase production was detected using the biochemical-based Carba NP test, based on the detection of in vitro hydrolysis of imipenem. All... More

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    Summary
    Objectives: Carbapenem-resistant Enterobacteriaceae isolates (n = 1485) were received at the French Associated National Reference Center for Antibiotic Resistance in 2012 and were characterized for their mechanism of resistance to carbapenems.Methods: Carbapenemase production was detected using the biochemical-based Carba NP test, based on the detection of in vitro hydrolysis of imipenem. All isolates with a positive Carba NP test result were characterized by PCR and sequencing.Results: Carbapenemase production was identified in 23.1% of the isolates. The main carbapenemase type identified was OXA-48 and derivatives (75.5%). An overseas source was clearly demonstrated for only 27.6% of the isolates.Conclusions: OXA-48 and derivatives are now the most prevalent carbapenemases in France, with a possible spread of these producers in the community