Systèmes industriels - Systemtechnik

Compact portable biosensor for arsenic detection in aqueous samples with Escherichia coli bioreporter cells

Truffer, Frederic ; Buffi, Nina ; Merulla, Davide ; Beggah, Siham ; van Lintel, Harald ; Renaud, Philippe ; van der Meer, Jan Roelof ; Geiser, Martial

In: Review of Scientific Instruments, 2014, vol. 85, no. 015120, p. 4

We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated... Mehr

Zum persönliche Liste hinzufügen
    Summary
    We present a compact portable biosensor to measure arsenic As(III) concentrations in water using Escherichia coli bioreporter cells. Escherichia coli expresses green fluorescent protein in a linearly dependent manner as a function of the arsenic concentration (between 0 and 100μg/L). The device accommodates a small polydimethylsiloxane microfluidic chip that holds the agarose-encapsulated bacteria, and a complete optical illumination/collection/detection system for automated quantitative fluorescence measurements. The device is capable of sampling water autonomously, controlling the whole measurement, storing and transmitting data over GSM networks. We demonstrate highly reproducible measurements of arsenic in drinking water at 10 and 50μg/L within 100 and 80 min, respectively.