Faculté des sciences

Nanofabrication of protein-patterned substrates for future cell adhesion experiments

Künzi, P.A. ; Lussi, J. ; Aeschimann, Laure ; Danuser, G. ; Textor, M. ; de Rooij, Nicolaas F. ; Staufer, Urs

In: Microelectronic Engineering, 2005, vol. 78-79, p. 582-586

A method for fabricating sub-micrometer size adhesion sites for future experiments in cell biology is presented. Glass substrates were coated with a thin layer of InSnO and SiO2. The SiO2 was structured by means of electron beam lithography and reactive ion etching, exposing sub-micrometer patches of the underlying InSnO. Dodecylphosphate, to which proteins can bind, was... Plus

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    Summary
    A method for fabricating sub-micrometer size adhesion sites for future experiments in cell biology is presented. Glass substrates were coated with a thin layer of InSnO and SiO2. The SiO2 was structured by means of electron beam lithography and reactive ion etching, exposing sub-micrometer patches of the underlying InSnO. Dodecylphosphate, to which proteins can bind, was selectively adsorbed on these InSnO structures, whereas poly-l-lysine-g-poly(ethylene glycol) was used to passivate the surrounding SiO2 against protein adsorption. The effectiveness of the process was investigated by fluorescent microscopy and scanning near-field optical microscopy on substrates which have been exposed to fluorescently labeled streptavidin.