Faculté des sciences

Structure of the Euglena gracilis chloroplast gene (psbA) coding for the 32-kDa protein of Photosystem II

Keller, Mario ; Stutz, Erhard

In: FEBS Letters, 1984, vol. 175, no. 1, p. 173-177

An EcoRI fragment from Euglena gracilis chloroplast DNA, called Eco.I (4.9 kbp), contains the gene for the ‘32-kDa’ thylakoid membrane protein of Photosystem II (psb A gene), the tRNA2Leu gene and additional sequences which possibly code for two proteins of unknown function. The transcription polarity is the same for all these coding sequences. The... Plus

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    Summary
    An EcoRI fragment from Euglena gracilis chloroplast DNA, called Eco.I (4.9 kbp), contains the gene for the ‘32-kDa’ thylakoid membrane protein of Photosystem II (psb A gene), the tRNA2Leu gene and additional sequences which possibly code for two proteins of unknown function. The transcription polarity is the same for all these coding sequences. The psb A gene is split: 4 introns (size range, 433–616 bp) separate 5 exons (size range, 39–579 bp) which code for a protein of Mr 38380 (345 amino acids). The Euglena protein is about 87% homologous with the higher plant counterparts but it contains 5 lysine residues.