Benzothiadiazole affects the leaf proteome in arctic bramble (Rubus arcticus)
- Hukkanen, Anne Department of Biosciences, University of Kuopio, Finland
- Kokko, Harri Department of Biosciences, University of Kuopio, Finland
- Buchala, Antony J. Department of Plant Biology, University of Fribourg, Switzerland
- Häyrinen, Jukka Department of Biosciences, University of Kuopio, Finland
- Kärenlampi, Sirpa Department of Biosciences, University of Kuopio, Finland
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19.08.2008
Published in:
- Molecular Plant Pathology. - 2008, vol. 9, no. 6, p. 799 - 808
English
Benzothiadiazole (BTH) induces resistance to the downy mildew pathogen, Peronospora sparsa, in arctic bramble, but the basis for the BTH-induced resistance is unknown. Arctic bramble cv. Mespi was treated with BTH to study the changes in leaf proteome and to identify proteins with a putative role in disease resistance. First, BTH induced strong expression of one PR-1 protein isoform, which was also induced by salicylic acid (SA). The PR-1 was responsive to BTH and exogenous SA despite a high endogenous SA content (20–25 µg/g fresh weight), which increased to an even higher level after treatment with BTH. Secondly, a total of 792 protein spots were detected in two-dimensional gel electrophoresis, eight proteins being detected solely in the BTH-treated plants. BTH caused up- or down-regulation of 72 and 31 proteins, respectively, of which 18 were tentatively identified by mass spectrometry. The up-regulation of flavanone-3-hydroxylase, alanine aminotransferase, 1-aminocyclopropane-1-carboxylate oxidase, PR-1 and PR-10 proteins may partly explain the BTH-induced resistance against P. sparsa. Other proteins with changes in intensity appear to be involved in, for example, energy metabolism and protein processing. The decline in ATP synthase, triosephosphate isomerase, fructose bisphosphate aldolase and glutamine synthetase suggests that BTH causes significant changes in primary metabolism, which provides one possible explanation for the decreased vegetative growth of foliage and rhizome observed in BTH-treated plants.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 10812
- DOI 10.1111/J.1364-3703.2008.00502.X
- Persistent URL
- https://folia.unifr.ch/unifr/documents/300931
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