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A transgenic dTph1 insertional mutagenesis system for forward genetics in mycorrhizal phosphate transport of Petunia

  • Wegmüller, Sarah HES-SO Valais Wallis, Institute of Life Technologies, Sion, Switzerland - ETH Zurich, Institute of Plant Sciences, Lindau, Switzerland
  • Svistoonoff, Sergio ETH Zurich, Institute of Plant Sciences, Lindau, Switzerland - Institut de Recherche pour le Developpement (IRD), Equipe Rhizogenese, Montpellierm France
  • Reinhardt, Didier Plant Biology, University of Fribourg, Switzerland
  • Stuurman, Jeroen Institute of Plant Sciences, University of Berne, Switzerland - KEYGENE NV, Wageningen, The Netherlands
  • Amrhein, Nikolaus ETH Zurich, Institute of Plant Sciences, Lindau, Switzerland - ETH Zurich, Institute of Plant Sciences, Switzerland
  • Bucher, Marcel ETH Zurich, Institute of Plant Sciences, Lindau, Switzerland - University of Cologne, Institute of Botany, Germany
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    08.05.2008
Published in:
  • The Plant Journal. - 2008, vol. 54, no. 6, p. 1115 - 1127
Petunia
forward genetics
transposon mutagenesis
mycorrhiza
phosphate transporter
luciferase
English The active endogenous dTph1 system of the Petunia hybrida mutator line W138 has been used in several forward-genetic mutant screens that were based on visible phenotypes such as flower morphology and color. In contrast, defective symbiotic phosphate (Pi) transport in mycorrhizal roots of Petunia is a hidden molecular phenotype as the symbiosis between plant roots and fungi takes place below ground, and, while fungal colonization can be visualized histochemically, Pi transport and the activity of Pi transporter proteins cannot be assessed visually. Here, we report on a molecular approach in which expression of a mycorrhiza-inducible bi-functional reporter transgene and insertional mutagenesis in Petunia are combined. Bi-directionalization of a mycorrhizal Pi transporter promoter controlling the expression of two reporter genes encoding firefly luciferase and GUS allows visualization of mycorrhiza-specific Pi transporter expression. A population of selectable transposon insertion mutants was established by crossing the transgenic reporter line with the mutator W138, from which the Pitransporter downregulated (ptd1) mutant was identified, which exhibits strongly reduced expression of mycorrhiza-inducible Pi transporters in mycorrhizal roots.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/300918
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